A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting
AuthorHicks, SM; Pohl, K; Neeman, T; McNamara, HA; Parsons, KM; He, J-S; Ali, SA; Nazir, S; Rowntree, LC; Nguyen, THO; ...
Source TitleJournal of Infectious Diseases
PublisherOXFORD UNIV PRESS INC
University of Melbourne Author/sRowntree, Louise; Kedzierska, Katherine; Nguyen, Thi Hoang Oanh; George, Amee
AffiliationMicrobiology and Immunology
Document TypeJournal Article
CitationsHicks, S. M., Pohl, K., Neeman, T., McNamara, H. A., Parsons, K. M., He, J. -S., Ali, S. A., Nazir, S., Rowntree, L. C., Nguyen, T. H. O., Kedzierska, K., Doolan, D. L., Vinuesa, C. G., Cook, M. C., Coatsworth, N., Myles, P. S., Kurth, F., Sander, L. E., Mann, G. J. ,... Cockburn, I. A. (2021). A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting. JOURNAL OF INFECTIOUS DISEASES, 223 (1), pp.10-14. https://doi.org/10.1093/infdis/jiaa623.
Access StatusAccess this item via the Open Access location
Open Access URLPublished version
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665523
Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.
- Click on "Export Reference in RIS Format" and choose "open with... Endnote".
- Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References