University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Microbiology & Immunology
  • Microbiology & Immunology - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Microbiology & Immunology
  • Microbiology & Immunology - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay.

    Thumbnail
    Citations
    Altmetric
    Author
    Graham, M; Williams, E; Isles, N; Buadromo, E; Toatu, T; Druce, J; Catton, M; Lin, C; Howden, BP; Williamson, DA
    Date
    2020-10-15
    Source Title
    Diagnostic Microbiology and Infectious Disease
    Publisher
    Elsevier BV
    University of Melbourne Author/s
    Howden, Benjamin; Graham, Maryza; Catton, Michael; Williamson, Deborah; Isles, Nicole; Druce, Julian; Lin, Chantel
    Affiliation
    Microbiology and Immunology
    Doherty Institute
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Graham, M., Williams, E., Isles, N., Buadromo, E., Toatu, T., Druce, J., Catton, M., Lin, C., Howden, B. P. & Williamson, D. A. (2020). Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay.. Diagn Microbiol Infect Dis, 99 (2), pp.115238-. https://doi.org/10.1016/j.diagmicrobio.2020.115238.
    Access Status
    Access this item via the Open Access location
    URI
    http://hdl.handle.net/11343/254582
    DOI
    10.1016/j.diagmicrobio.2020.115238
    Open Access URL
    https://europepmc.org/articles/PMC7558241?pdf=render
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558241
    Abstract
    The COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples in pools of 4 and 6. Clinical samples containing SARS-CoV-2, and confirmed negative clinical samples were used to create sample pools. Clinical samples had 'neat' Xpert® E gene cycle threshold values ranging between 20 and 28 and all were detected qualitatively when contained in pools of 4 or 6 samples. For these samples, pooling had a median change in cycle threshold value of 2.0 in pools of 4, and of 2.9 in pools of 6. With the use of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay, pooling of 4 or 6 samples may be an effective strategy to increase testing capacity.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [45689]
    • Doherty Institute - Research Publications [371]
    • Microbiology & Immunology - Research Publications [1555]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors