Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay.
AuthorGraham, M; Williams, E; Isles, N; Buadromo, E; Toatu, T; Druce, J; Catton, M; Lin, C; Howden, BP; Williamson, DA
Source TitleDiagnostic Microbiology and Infectious Disease
University of Melbourne Author/sHowden, Benjamin; Graham, Maryza; Catton, Michael; Williamson, Deborah; Isles, Nicole; Druce, Julian; Lin, Chantel
AffiliationMicrobiology and Immunology
Document TypeJournal Article
CitationsGraham, M., Williams, E., Isles, N., Buadromo, E., Toatu, T., Druce, J., Catton, M., Lin, C., Howden, B. P. & Williamson, D. A. (2020). Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay.. Diagn Microbiol Infect Dis, 99 (2), pp.115238-. https://doi.org/10.1016/j.diagmicrobio.2020.115238.
Access StatusAccess this item via the Open Access location
Open Access URLhttps://europepmc.org/articles/PMC7558241?pdf=render
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558241
The COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples in pools of 4 and 6. Clinical samples containing SARS-CoV-2, and confirmed negative clinical samples were used to create sample pools. Clinical samples had 'neat' Xpert® E gene cycle threshold values ranging between 20 and 28 and all were detected qualitatively when contained in pools of 4 or 6 samples. For these samples, pooling had a median change in cycle threshold value of 2.0 in pools of 4, and of 2.9 in pools of 6. With the use of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay, pooling of 4 or 6 samples may be an effective strategy to increase testing capacity.
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