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dc.contributor.authorGraham, M
dc.contributor.authorWilliams, E
dc.contributor.authorIsles, N
dc.contributor.authorBuadromo, E
dc.contributor.authorToatu, T
dc.contributor.authorDruce, J
dc.contributor.authorCatton, M
dc.contributor.authorLin, C
dc.contributor.authorHowden, BP
dc.contributor.authorWilliamson, DA
dc.date.accessioned2020-12-16T23:50:20Z
dc.date.available2020-12-16T23:50:20Z
dc.date.issued2021-02-01
dc.identifierpii: S0732-8893(20)30615-5
dc.identifier.citationGraham, M., Williams, E., Isles, N., Buadromo, E., Toatu, T., Druce, J., Catton, M., Lin, C., Howden, B. P. & Williamson, D. A. (2021). Sample pooling on the Cepheid Xpert (R) Xpress SARS-CoV-2 assay. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 99 (2), https://doi.org/10.1016/j.diagmicrobio.2020.115238.
dc.identifier.issn0732-8893
dc.identifier.urihttp://hdl.handle.net/11343/254582
dc.description.abstractThe COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples in pools of 4 and 6. Clinical samples containing SARS-CoV-2, and confirmed negative clinical samples were used to create sample pools. Clinical samples had 'neat' Xpert® E gene cycle threshold values ranging between 20 and 28 and all were detected qualitatively when contained in pools of 4 or 6 samples. For these samples, pooling had a median change in cycle threshold value of 2.0 in pools of 4, and of 2.9 in pools of 6. With the use of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay, pooling of 4 or 6 samples may be an effective strategy to increase testing capacity.
dc.languageEnglish
dc.publisherELSEVIER SCIENCE INC
dc.titleSample pooling on the Cepheid Xpert (R) Xpress SARS-CoV-2 assay
dc.typeJournal Article
dc.identifier.doi10.1016/j.diagmicrobio.2020.115238
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.departmentDoherty Institute
melbourne.source.titleDiagnostic Microbiology and Infectious Disease
melbourne.source.volume99
melbourne.source.issue2
melbourne.source.pages115238-
melbourne.elementsid1468900
melbourne.openaccess.urlhttps://europepmc.org/articles/PMC7558241?pdf=render
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558241
melbourne.openaccess.statusPublished version
melbourne.contributor.authorHowden, Benjamin
melbourne.contributor.authorGraham, Maryza
melbourne.contributor.authorCatton, Michael
melbourne.contributor.authorWilliamson, Deborah
melbourne.contributor.authorIsles, Nicole
melbourne.contributor.authorDruce, Julian
melbourne.contributor.authorLin, Chantel
dc.identifier.eissn1879-0070
melbourne.accessrightsAccess this item via the Open Access location


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