Myeloid-derived miR-223 regulates intestinal inflammation via repression of the NLRP3 inflammasome
AuthorNeudecker, V; Haneklaus, M; Jensen, O; Khailova, L; Masterson, JC; Tye, H; Biette, K; Jedlicka, P; Brodsky, KS; Gerich, ME; ...
Source TitleJournal of Experimental Medicine
PublisherROCKEFELLER UNIV PRESS
University of Melbourne Author/sMasters, Seth
AffiliationMedical Biology (W.E.H.I.)
Document TypeJournal Article
CitationsNeudecker, V., Haneklaus, M., Jensen, O., Khailova, L., Masterson, J. C., Tye, H., Biette, K., Jedlicka, P., Brodsky, K. S., Gerich, M. E., Mack, M., Robertson, A. A. B., Cooper, M. A., Furuta, G. T., Dinarello, C. A., O'Neill, L. A., Eltzschig, H. K., Masters, S. L. & McNamee, E. N. (2017). Myeloid-derived miR-223 regulates intestinal inflammation via repression of the NLRP3 inflammasome. JOURNAL OF EXPERIMENTAL MEDICINE, 214 (6), pp.1737-1752. https://doi.org/10.1084/jem.20160462.
Access StatusOpen Access
MicroRNA (miRNA)-mediated RNA interference regulates many immune processes, but how miRNA circuits orchestrate aberrant intestinal inflammation during inflammatory bowel disease (IBD) is poorly defined. Here, we report that miR-223 limits intestinal inflammation by constraining the nlrp3 inflammasome. miR-223 was increased in intestinal biopsies from patients with active IBD and in preclinical models of intestinal inflammation. miR-223-/y mice presented with exacerbated myeloid-driven experimental colitis with heightened clinical, histopathological, and cytokine readouts. Mechanistically, enhanced NLRP3 inflammasome expression with elevated IL-1β was a predominant feature during the initiation of colitis with miR-223 deficiency. Depletion of CCR2+ inflammatory monocytes and pharmacologic blockade of IL-1β or NLRP3 abrogated this phenotype. Generation of a novel mouse line, with deletion of the miR-223 binding site in the NLRP3 3' untranslated region, phenocopied the characteristics of miR-223-/y mice. Finally, nanoparticle-mediated overexpression of miR-223 attenuated experimental colitis, NLRP3 levels, and IL-1β release. Collectively, our data reveal a previously unappreciated role for miR-223 in regulating the innate immune response during intestinal inflammation.
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