Serological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.

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Chen, MI; Barr, IG; Koh, GCH; Lee, VJ; Lee, CPS; Shaw, R; Lin, C; Yap, J; Cook, AR; Tan, BH; ...Date
2010-08-30Source Title
PLoS OnePublisher
Public Library of Science (PLoS)University of Melbourne Author/s
Barr, IanAffiliation
Microbiology and ImmunologyMetadata
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Chen, M. I., Barr, I. G., Koh, G. C. H., Lee, V. J., Lee, C. P. S., Shaw, R., Lin, C., Yap, J., Cook, A. R., Tan, B. H., Loh, J. P., Barkham, T., Chow, V. T. K., Lin, R. T. P. & Leo, Y. -S. (2010). Serological response in RT-PCR confirmed H1N1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: an observational study.. PLoS One, 5 (8), pp.e12474-. https://doi.org/10.1371/journal.pone.0012474.Access Status
Open AccessOpen Access at PMC
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2930007Abstract
BACKGROUND: We describe the serological response following H1N1-2009 influenza A infections confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). METHODOLOGY AND PRINCIPAL FINDINGS: The study included patients admitted to hospital, subjects of a seroepidemiologic cohort study, and participants identified from outbreak studies in Singapore. Baseline (first available blood sample) and follow-up blood samples were analyzed for antibody titers to H1N1-2009 and recently circulating seasonal influenza A virus strains by hemagglutination inhibition (HI) and virus micro-neutralization (VM) assays. 267 samples from 118 cases of H1N1-2009 were analyzed. Geometric mean titers by HI peaked at 123 (95% confidence interval, CI 43-356) between days 30 to 39. The chance of observing seroconversion (four-fold or greater increase of antibodies) was maximized when restricting analysis to 45 participants with baseline sera collected within 5 days of onset and follow-up sera collected 15 or more days after onset; for these participants, 82% and 89% seroconverted to A/California/7/2009 H1N1 by HI and VM respectively. A four-fold or greater increase in cross-reactive antibody titers to seasonal A/Brisbane/59/2007 H1N1, A/Brisbane/10/2007 H3N2 and A/Wisconsin/15/2009 H3N2 occurred in 20%, 18% and 16% of participants respectively. CONCLUSIONS AND SIGNIFICANCE: Appropriately timed paired serology detects 80-90% RT-PCR confirmed H1N1-2009; Antibodies from infection with H1N1-2009 cross-reacted with seasonal influenza viruses.
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