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dc.contributor.authorPayne, SC
dc.contributor.authorShepherd, RK
dc.contributor.authorSedo, A
dc.contributor.authorFallon, JB
dc.contributor.authorFurness, JB
dc.date.accessioned2020-12-17T02:53:03Z
dc.date.available2020-12-17T02:53:03Z
dc.date.issued2018-03-01
dc.identifierpii: rsos180107
dc.identifier.citationPayne, S. C., Shepherd, R. K., Sedo, A., Fallon, J. B. & Furness, J. B. (2018). An objective in vivo diagnostic method for inflammatory bowel disease. ROYAL SOCIETY OPEN SCIENCE, 5 (3), https://doi.org/10.1098/rsos.180107.
dc.identifier.issn2054-5703
dc.identifier.urihttp://hdl.handle.net/11343/254657
dc.description.abstractInflammatory damage to the bowel, as occurs in inflammatory bowel disease (IBD), is debilitating to patients. In both patients and animal experimental models, histological analyses of biopsies and endoscopic examinations are used to evaluate the disease state. However, such measurements often have delays and are invasive, while endoscopy is not quantitatively objective. Therefore, a real-time quantitative method to assess compromised mucosal barrier function is advantageous. We investigated the correlation of in vivo changes in electrical transmural impedance with histological measures of inflammation. Four platinum (Pt) ball electrodes were placed in the lumen of the rat small intestine, with a return electrode under the skin. Electrodes placed within the non-inflamed intestine generated stable impedances during the 3 h testing period. Following an intraluminal injection of 2,4,6-trinitrobenzene sulfonic acid (TNBS), an established animal model of IBD, impedances in the inflamed region significantly decreased relative to a region not exposed to TNBS (p < 0.05). Changes in intestinal transmural impedance were correlated (p < 0.05) with histologically assessed damage to the mucosa and increases in neutrophil, eosinophil and T-cell populations at 3 h compared with tissue from control regions. This quantitative, real-time assay may have application in the diagnosis and clinical management of IBD.
dc.languageEnglish
dc.publisherROYAL SOC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleAn objective in vivo diagnostic method for inflammatory bowel disease
dc.typeJournal Article
dc.identifier.doi10.1098/rsos.180107
melbourne.affiliation.departmentAnatomy and Neuroscience
melbourne.affiliation.departmentMedical Bionics
melbourne.affiliation.departmentFlorey Department of Neuroscience and Mental Health
melbourne.source.titleRoyal Society Open Science
melbourne.source.volume5
melbourne.source.issue3
dc.rights.licenseCC BY
melbourne.elementsid1321279
melbourne.contributor.authorFurness, John
melbourne.contributor.authorShepherd, Robert
melbourne.contributor.authorFallon, James
melbourne.contributor.authorPayne, Sophie
melbourne.contributor.authorSedo, Alicia
dc.identifier.eissn2054-5703
melbourne.accessrightsOpen Access


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