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dc.contributor.authorMoslehi, M
dc.contributor.authorNg, DCH
dc.contributor.authorBogoyevitch, MA
dc.date.accessioned2020-12-17T02:58:02Z
dc.date.available2020-12-17T02:58:02Z
dc.date.issued2017-07-12
dc.identifierpii: 10.1038/s41598-017-05340-x
dc.identifier.citationMoslehi, M., Ng, D. C. H. & Bogoyevitch, M. A. (2017). Dynamic microtubule association of Doublecortin X (DCX) is regulated by its C-terminus. SCIENTIFIC REPORTS, 7 (1), https://doi.org/10.1038/s41598-017-05340-x.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/254693
dc.description.abstractDoublecortin X (DCX), known to be essential for neuronal migration and cortical layering in the developing brain, is a 40 kDa microtubule (MT)-associated protein. DCX directly interacts with MTs via its two structured doublecortin (DC) domains, but the dynamics of this association and the possible regulatory roles played by the flanking unstructured regions remain poorly defined. Here, we employ quantitative fluorescence recovery after photobleaching (FRAP) protocols in living cells to reveal that DCX shows remarkably rapid and complete exchange within the MT network but that the removal of the C-terminal region significantly slows this exchange. We further probed how MT organization or external stimuli could additionally modulate DCX exchange dynamics. MT depolymerisation (nocodazole treatment) or stabilization (taxol treatment) further enhanced DCX exchange rates, however the exchange rates for the C-terminal truncated DCX protein were resistant to the impact of taxol-induced stabilization. Furthermore, in response to a hyperosmotic stress stimulus, DCX exchange dynamics were slowed, and again the C-terminal truncated DCX protein was resistant to the stimulus. Thus, the DCX dynamically associates with MTs in living cells and its C-terminal region plays important roles in the MT-DCX association.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleDynamic microtubule association of Doublecortin X (DCX) is regulated by its C-terminus
dc.typeJournal Article
dc.identifier.doi10.1038/s41598-017-05340-x
melbourne.affiliation.departmentBiochemistry and Molecular Biology
melbourne.source.titleScientific Reports
melbourne.source.volume7
melbourne.source.issue1
melbourne.identifier.arcFT120100193
dc.rights.licenseCC BY
melbourne.elementsid1221528
melbourne.contributor.authorMoslehi, Maryam
melbourne.contributor.authorNg, Dominic
melbourne.contributor.authorBogoyevitch, Marie
dc.identifier.eissn2045-2322
melbourne.identifier.fundernameidAUST RESEARCH COUNCIL, FT120100193
melbourne.accessrightsOpen Access


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