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dc.contributor.authorDeo, P
dc.contributor.authorChow, SH
dc.contributor.authorHay, ID
dc.contributor.authorKleifeld, O
dc.contributor.authorCostin, A
dc.contributor.authorElgass, KD
dc.contributor.authorJiang, J-H
dc.contributor.authorRamm, G
dc.contributor.authorGabriel, K
dc.contributor.authorDougan, G
dc.contributor.authorLithgow, T
dc.contributor.authorHeinz, E
dc.contributor.authorNaderer, T
dc.date.accessioned2020-12-17T03:05:37Z
dc.date.available2020-12-17T03:05:37Z
dc.date.issued2018-03-01
dc.identifierpii: PPATHOGENS-D-17-01609
dc.identifier.citationDeo, P., Chow, S. H., Hay, I. D., Kleifeld, O., Costin, A., Elgass, K. D., Jiang, J. -H., Ramm, G., Gabriel, K., Dougan, G., Lithgow, T., Heinz, E. & Naderer, T. (2018). Outer membrane vesicles from Neisseria gonorrhoeae target PorB to mitochondria and induce apoptosis. PLOS PATHOGENS, 14 (3), https://doi.org/10.1371/journal.ppat.1006945.
dc.identifier.issn1553-7366
dc.identifier.urihttp://hdl.handle.net/11343/254740
dc.description.abstractNeisseria gonorrhoeae causes the sexually transmitted disease gonorrhoea by evading innate immunity. Colonizing the mucosa of the reproductive tract depends on the bacterial outer membrane porin, PorB, which is essential for ion and nutrient uptake. PorB is also targeted to host mitochondria and regulates apoptosis pathways to promote infections. How PorB traffics from the outer membrane of N. gonorrhoeae to mitochondria and whether it modulates innate immune cells, such as macrophages, remains unclear. Here, we show that N. gonorrhoeae secretes PorB via outer membrane vesicles (OMVs). Purified OMVs contained primarily outer membrane proteins including oligomeric PorB. The porin was targeted to mitochondria of macrophages after exposure to purified OMVs and wild type N. gonorrhoeae. This was associated with loss of mitochondrial membrane potential, release of cytochrome c, activation of apoptotic caspases and cell death in a time-dependent manner. Consistent with this, OMV-induced macrophage death was prevented with the pan-caspase inhibitor, Q-VD-PH. This shows that N. gonorrhoeae utilizes OMVs to target PorB to mitochondria and to induce apoptosis in macrophages, thus affecting innate immunity.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleOuter membrane vesicles from Neisseria gonorrhoeae target PorB to mitochondria and induce apoptosis
dc.typeJournal Article
dc.identifier.doi10.1371/journal.ppat.1006945
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titlePLoS Pathogens
melbourne.source.volume14
melbourne.source.issue3
dc.rights.licenseCC BY
melbourne.elementsid1319544
melbourne.contributor.authorDougan, Gordon
melbourne.contributor.authorJiang, Jhih-Hang
dc.identifier.eissn1553-7374
melbourne.accessrightsOpen Access


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