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dc.contributor.authorBalloch, A
dc.contributor.authorRoalfe, L
dc.contributor.authorEkstrom, N
dc.contributor.authorNguyen, CD
dc.contributor.authorSpry, L
dc.contributor.authorMarimla, RA
dc.contributor.authorLicciardi, PV
dc.contributor.authorGoldblatt, D
dc.contributor.authorMulholland, EK
dc.date.accessioned2020-12-17T03:28:08Z
dc.date.available2020-12-17T03:28:08Z
dc.date.issued2018-04-25
dc.identifierpii: 3/2/e00070-18
dc.identifier.citationBalloch, A., Roalfe, L., Ekstrom, N., Nguyen, C. D., Spry, L., Marimla, R. A., Licciardi, P. V., Goldblatt, D. & Mulholland, E. K. (2018). Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera.. mSphere, 3 (2), pp.e00070-e00018. https://doi.org/10.1128/mSphere.00070-18.
dc.identifier.issn2379-5042
dc.identifier.urihttp://hdl.handle.net/11343/254899
dc.description.abstractOpsonophagocytic assays are used to measure functional antibodies important in protection against pneumococcal capsular antigens. There have been efforts to standardize these methods, as the assays are commonly used to measure vaccine immunogenicity. We report here the results from three international laboratories using their own methods, based on the recommended WHO standard method. We tested 30 pediatric sera, before and after administration of a 13-valent conjugate pneumococcal vaccine, against all 13 serotypes. The three laboratories demonstrated good agreement using their own standardized multiplex opsonophagocytosis assay protocols, particularly postimmunization for those serotypes in the vaccine. While serotype-specific IgG methods have already been internationally standardized and are currently used as a measure of vaccine immunogenicity, this report demonstrates that despite minor differences in methods and a minor variation in response to nonvaccine serotypes, the results from opsonophagocytic assays across the three laboratories may be compared with confidence.IMPORTANCE When measuring a functional antibody response to pneumococcal immunization, it is imperative that a specific, reproducible, accurate, and standardized assay with acceptable inter- and intra-assay variation be advocated internationally to allow for meaningful comparison of results between laboratories. We report here the results of a collaboration between 3 international laboratories testing 30 pediatric samples against the 13 serotypes in Prevenar13.
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.titleInterlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera.
dc.typeJournal Article
dc.identifier.doi10.1128/mSphere.00070-18
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.source.titlemSphere
melbourne.source.volume3
melbourne.source.issue2
melbourne.source.pagese00070-e00018
dc.rights.licenseCC BY
melbourne.elementsid1325856
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917425
melbourne.contributor.authorMulholland, Edward
melbourne.contributor.authorLicciardi, Paul
melbourne.contributor.authorNguyen, Cattram
dc.identifier.eissn2379-5042
melbourne.accessrightsOpen Access


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