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    Analysis of Mitochondrial Function and Localisation during Human Embryonic Stem Cell Differentiation In Vitro

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    Author
    Prowse, ABJ; Chong, F; Elliott, DA; Elefanty, AG; Stanley, EG; Gray, PP; Munro, TP; Osborne, GW
    Date
    2012-12-19
    Source Title
    PLoS One
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Stanley, Edouard; Elefanty, Andrew
    Affiliation
    Paediatrics (RCH)
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Prowse, A. B. J., Chong, F., Elliott, D. A., Elefanty, A. G., Stanley, E. G., Gray, P. P., Munro, T. P. & Osborne, G. W. (2012). Analysis of Mitochondrial Function and Localisation during Human Embryonic Stem Cell Differentiation In Vitro. PLOS ONE, 7 (12), https://doi.org/10.1371/journal.pone.0052214.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/254947
    DOI
    10.1371/journal.pone.0052214
    Abstract
    Human embryonic stem cell (hESC) derivatives show promise as viable cell therapy options for multiple disorders in different tissues. Recent advances in stem cell biology have lead to the reliable production and detailed molecular characterisation of a range of cell-types. However, the role of mitochondria during differentiation has yet to be fully elucidated. Mitochondria mediate a cells response to altered energy requirements (e.g. cardiomyocyte contraction) and, as such, the mitochondrial phenotype is likely to change during the dynamic process of hESC differentiation. We demonstrate that manipulating mitochondrial biogenesis alters mesendoderm commitment. To investigate mitochondrial localisation during early lineage specification of hESCs we developed a mitochondrial reporter line, KMEL2, in which sequences encoding the green fluorescent protein (GFP) are targeted to the mitochondria. Differentiation of KMEL2 lines into the three germ layers showed that the mitochondria in these differentiated progeny are GFP positive. Therefore, KMEL2 hESCs facilitate the study of mitochondria in a range of cell types and, importantly, permit real-time analysis of mitochondria via the GFP tag.

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