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dc.contributor.authorTan, JL
dc.contributor.authorLau, SN
dc.contributor.authorLeaw, B
dc.contributor.authorNguyen, HPT
dc.contributor.authorSalamonsen, LA
dc.contributor.authorSaad, MI
dc.contributor.authorChan, ST
dc.contributor.authorZhu, D
dc.contributor.authorKrause, M
dc.contributor.authorKim, C
dc.contributor.authorSievert, W
dc.contributor.authorWallace, EM
dc.contributor.authorLim, R
dc.date.accessioned2020-12-17T03:36:05Z
dc.date.available2020-12-17T03:36:05Z
dc.date.issued2018-02-01
dc.identifier.citationTan, J. L., Lau, S. N., Leaw, B., Nguyen, H. P. T., Salamonsen, L. A., Saad, M. I., Chan, S. T., Zhu, D., Krause, M., Kim, C., Sievert, W., Wallace, E. M. & Lim, R. (2018). Amnion Epithelial Cell-Derived Exosomes Restrict Lung Injury and Enhance Endogenous Lung Repair. STEM CELLS TRANSLATIONAL MEDICINE, 7 (2), pp.180-196. https://doi.org/10.1002/sctm.17-0185.
dc.identifier.issn2157-6564
dc.identifier.urihttp://hdl.handle.net/11343/254952
dc.description.abstractIdiopathic pulmonary fibrosis (IPF) is characterized by chronic inflammation, severe scarring, and stem cell senescence. Stem cell-based therapies modulate inflammatory and fibrogenic pathways by release of soluble factors. Stem cell-derived extracellular vesicles should be explored as a potential therapy for IPF. Human amnion epithelial cell-derived exosomes (hAEC Exo) were isolated and compared against human lung fibroblasts exosomes. hAEC Exo were assessed as a potential therapy for lung fibrosis. Exosomes were isolated and evaluated for their protein and miRNA cargo. Direct effects of hAEC Exo on immune cell function, including macrophage polarization, phagocytosis, neutrophil myeloperoxidase activity and T cell proliferation and uptake, were measured. Their impact on immune response, histological outcomes, and bronchioalveolar stem cell (BASC) response was assessed in vivo following bleomycin challenge in young and aged mice. hAEC Exo carry protein cargo enriched for MAPK signaling pathways, apoptotic and developmental biology pathways and miRNA enriched for PI3K-Akt, Ras, Hippo, TGFβ, and focal adhesion pathways. hAEC Exo polarized and increased macrophage phagocytosis, reduced neutrophil myeloperoxidases, and suppressed T cell proliferation directly. Intranasal instillation of 10 μg hAEC Exo 1 day following bleomycin challenge reduced lung inflammation, while treatment at day 7 improved tissue-to-airspace ratio and reduced fibrosis. Administration of hAEC Exo coincided with the proliferation of BASC. These effects were reproducible in bleomycin-challenged aged mice. The paracrine effects of hAECs can be largely attributed to their exosomes and exploitation of hAEC Exo as a therapy for IPF should be explored further. Stem Cells Translational Medicine 2018;7:180-196.
dc.languageEnglish
dc.publisherWILEY
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.titleAmnion Epithelial Cell-Derived Exosomes Restrict Lung Injury and Enhance Endogenous Lung Repair
dc.typeJournal Article
dc.identifier.doi10.1002/sctm.17-0185
melbourne.affiliation.departmentSurgery (RMH)
melbourne.source.titleStem Cells Translational Medicine
melbourne.source.volume7
melbourne.source.issue2
melbourne.source.pages180-196
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1326308
melbourne.contributor.authorNguyen, Hong
dc.identifier.eissn2157-6580
melbourne.accessrightsOpen Access


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