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    Bacteroides thetaiotaomicron generates diverse alpha-mannosidase activities through subtle evolution of a distal substrate-binding motif

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    Author
    Thompson, AJ; Spears, RJ; Zhu, Y; Suits, MDL; Williams, SJ; Gilbert, HJ; Davies, GJ
    Date
    2018-05-01
    Source Title
    Acta crystallographica. Section D, Structural biology
    Publisher
    INT UNION CRYSTALLOGRAPHY
    University of Melbourne Author/s
    Williams, Spencer
    Affiliation
    School of Chemistry
    Metadata
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    Document Type
    Journal Article
    Citations
    Thompson, A. J., Spears, R. J., Zhu, Y., Suits, M. D. L., Williams, S. J., Gilbert, H. J. & Davies, G. J. (2018). Bacteroides thetaiotaomicron generates diverse alpha-mannosidase activities through subtle evolution of a distal substrate-binding motif. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 74 (Pt 5), pp.394-404. https://doi.org/10.1107/S2059798318002942.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/254959
    DOI
    10.1107/S2059798318002942
    Abstract
    A dominant human gut microbe, the well studied symbiont Bacteroides thetaiotaomicron (Bt), is a glyco-specialist that harbors a large repertoire of genes devoted to carbohydrate processing. Despite strong similarities among them, many of the encoded enzymes have evolved distinct substrate specificities, and through the clustering of cognate genes within operons termed polysaccharide-utilization loci (PULs) enable the fulfilment of complex biological roles. Structural analyses of two glycoside hydrolase family 92 α-mannosidases, BT3130 and BT3965, together with mechanistically relevant complexes at 1.8-2.5 Å resolution reveal conservation of the global enzyme fold and core catalytic apparatus despite different linkage specificities. Structure comparison shows that Bt differentiates the activity of these enzymes through evolution of a highly variable substrate-binding region immediately adjacent to the active site. These observations unveil a genetic/biochemical mechanism through which polysaccharide-processing bacteria can evolve new and specific biochemical activities from otherwise highly similar gene products.

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