Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

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Awel, S; Kirian, RA; Wiedorn, MO; Beyerlein, KR; Roth, N; Horke, DA; Oberthuer, D; Knoska, J; Mariani, V; Morgan, A; ...Date
2018-02-01Source Title
Journal of Applied CrystallographyPublisher
INT UNION CRYSTALLOGRAPHYUniversity of Melbourne Author/s
Morgan, AndrewAffiliation
School of PhysicsMetadata
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Journal ArticleCitations
Awel, S., Kirian, R. A., Wiedorn, M. O., Beyerlein, K. R., Roth, N., Horke, D. A., Oberthuer, D., Knoska, J., Mariani, V., Morgan, A., Adriano, L., Tolstikova, A., Xavier, P. L., Yefanov, O., Aquila, A., Barty, A., Roy-Chowdhury, S., Hunter, M. S., James, D. ,... Chapman, H. N. (2018). Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 51 (Pt 1), pp.133-139. https://doi.org/10.1107/S1600576717018131.Access Status
Open AccessAbstract
High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.
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