Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals
Web of Science
AuthorAwel, S; Kirian, RA; Wiedorn, MO; Beyerlein, KR; Roth, N; Horke, DA; Oberthuer, D; Knoska, J; Mariani, V; Morgan, A; ...
Source TitleJournal of Applied Crystallography
PublisherINT UNION CRYSTALLOGRAPHY
University of Melbourne Author/sMorgan, Andrew
AffiliationSchool of Physics
Document TypeJournal Article
CitationsAwel, S., Kirian, R. A., Wiedorn, M. O., Beyerlein, K. R., Roth, N., Horke, D. A., Oberthuer, D., Knoska, J., Mariani, V., Morgan, A., Adriano, L., Tolstikova, A., Xavier, P. L., Yefanov, O., Aquila, A., Barty, A., Roy-Chowdhury, S., Hunter, M. S., James, D. ,... Chapman, H. N. (2018). Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 51 (Pt 1), pp.133-139. https://doi.org/10.1107/S1600576717018131.
Access StatusOpen Access
High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.
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