Show simple item record

dc.contributor.authorCuiv, PO
dc.contributor.authorGiri, R
dc.contributor.authorHoedt, EC
dc.contributor.authorMcGuckin, MA
dc.contributor.authorBegun, J
dc.contributor.authorMorrison, M
dc.date.accessioned2020-12-17T03:42:01Z
dc.date.available2020-12-17T03:42:01Z
dc.date.issued2018-04-18
dc.identifier.citationCuiv, P. O., Giri, R., Hoedt, E. C., McGuckin, M. A., Begun, J. & Morrison, M. (2018). &ITEnterococcus faecalis&IT AHG0090 is a Genetically Tractable Bacterium and Produces a Secreted Peptidic Bioactive that Suppresses Nuclear Factor Kappa B Activation in Human Gut Epithelial Cells. FRONTIERS IN IMMUNOLOGY, 9 (APR), https://doi.org/10.3389/fimmu.2018.00790.
dc.identifier.issn1664-3224
dc.identifier.urihttp://hdl.handle.net/11343/254994
dc.description.abstractEnterococcus faecalis is an early coloniser of the human infant gut and contributes to the development of intestinal immunity. To better understand the functional capacity of E. faecalis, we constructed a broad host range RP4 mobilizable vector, pEHR513112, that confers chloramphenicol resistance and used a metaparental mating approach to isolate E. faecalis AHG0090 from a fecal sample collected from a healthy human infant. We demonstrated that E. faecalis AHG0090 is genetically tractable and could be manipulated using traditional molecular microbiology approaches. E. faecalis AHG0090 was comparable to the gold-standard anti-inflammatory bacterium Faecalibacterium prausnitzii A2-165 in its ability to suppress cytokine-mediated nuclear factor kappa B (NF-κB) activation in human gut-derived LS174T goblet cell like and Caco-2 enterocyte-like cell lines. E. faecalis AHG0090 and F. prausnitzii A2-165 produced secreted low molecular weight NF-κB suppressive peptidic bioactives. Both bioactives were sensitive to heat and proteinase K treatments although the E. faecalis AHG0090 bioactive was more resilient to both forms of treatment. As expected, E. faecalis AHG0090 suppressed IL-1β-induced NF-κB-p65 subunit nuclear translocation and expression of the NF-κB regulated genes IL-6, IL-8 and CXCL-10. Finally, we determined that E. faecalis AHG0090 is distantly related to other commensal strains and likely encodes niche factors that support effective colonization of the infant gut.
dc.languageEnglish
dc.publisherFRONTIERS MEDIA SA
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.title&ITEnterococcus faecalis&IT AHG0090 is a Genetically Tractable Bacterium and Produces a Secreted Peptidic Bioactive that Suppresses Nuclear Factor Kappa B Activation in Human Gut Epithelial Cells
dc.typeJournal Article
dc.identifier.doi10.3389/fimmu.2018.00790
melbourne.affiliation.departmentMedicine Dentistry & Health Sciences
melbourne.source.titleFrontiers in Immunology
melbourne.source.volume9
melbourne.source.issueAPR
dc.rights.licenseCC BY
melbourne.elementsid1326553
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915459
melbourne.contributor.authorMcGuckin, Michael
dc.identifier.eissn1664-3224
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record