dc.contributor.author | Cuiv, PO | |
dc.contributor.author | Giri, R | |
dc.contributor.author | Hoedt, EC | |
dc.contributor.author | McGuckin, MA | |
dc.contributor.author | Begun, J | |
dc.contributor.author | Morrison, M | |
dc.date.accessioned | 2020-12-17T03:42:01Z | |
dc.date.available | 2020-12-17T03:42:01Z | |
dc.date.issued | 2018-04-18 | |
dc.identifier.citation | Cuiv, P. O., Giri, R., Hoedt, E. C., McGuckin, M. A., Begun, J. & Morrison, M. (2018). &ITEnterococcus faecalis&IT AHG0090 is a Genetically Tractable Bacterium and Produces a Secreted Peptidic Bioactive that Suppresses Nuclear Factor Kappa B Activation in Human Gut Epithelial Cells. FRONTIERS IN IMMUNOLOGY, 9 (APR), https://doi.org/10.3389/fimmu.2018.00790. | |
dc.identifier.issn | 1664-3224 | |
dc.identifier.uri | http://hdl.handle.net/11343/254994 | |
dc.description.abstract | Enterococcus faecalis is an early coloniser of the human infant gut and contributes to the development of intestinal immunity. To better understand the functional capacity of E. faecalis, we constructed a broad host range RP4 mobilizable vector, pEHR513112, that confers chloramphenicol resistance and used a metaparental mating approach to isolate E. faecalis AHG0090 from a fecal sample collected from a healthy human infant. We demonstrated that E. faecalis AHG0090 is genetically tractable and could be manipulated using traditional molecular microbiology approaches. E. faecalis AHG0090 was comparable to the gold-standard anti-inflammatory bacterium Faecalibacterium prausnitzii A2-165 in its ability to suppress cytokine-mediated nuclear factor kappa B (NF-κB) activation in human gut-derived LS174T goblet cell like and Caco-2 enterocyte-like cell lines. E. faecalis AHG0090 and F. prausnitzii A2-165 produced secreted low molecular weight NF-κB suppressive peptidic bioactives. Both bioactives were sensitive to heat and proteinase K treatments although the E. faecalis AHG0090 bioactive was more resilient to both forms of treatment. As expected, E. faecalis AHG0090 suppressed IL-1β-induced NF-κB-p65 subunit nuclear translocation and expression of the NF-κB regulated genes IL-6, IL-8 and CXCL-10. Finally, we determined that E. faecalis AHG0090 is distantly related to other commensal strains and likely encodes niche factors that support effective colonization of the infant gut. | |
dc.language | English | |
dc.publisher | FRONTIERS MEDIA SA | |
dc.title | &ITEnterococcus faecalis&IT AHG0090 is a Genetically Tractable Bacterium and Produces a Secreted Peptidic Bioactive that Suppresses Nuclear Factor Kappa B Activation in Human Gut Epithelial Cells | |
dc.type | Journal Article | |
dc.identifier.doi | 10.3389/fimmu.2018.00790 | |
melbourne.affiliation.department | Medicine Dentistry & Health Sciences | |
melbourne.source.title | Frontiers in Immunology | |
melbourne.source.volume | 9 | |
melbourne.source.issue | APR | |
dc.rights.license | CC BY | |
melbourne.elementsid | 1326553 | |
melbourne.openaccess.pmc | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915459 | |
melbourne.contributor.author | McGuckin, Michael | |
dc.identifier.eissn | 1664-3224 | |
melbourne.accessrights | Open Access | |