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dc.contributor.authorWahlgren, WY
dc.contributor.authorDunevall, E
dc.contributor.authorNorth, RA
dc.contributor.authorPaz, A
dc.contributor.authorScalise, M
dc.contributor.authorBisignano, P
dc.contributor.authorBengtsson-Palme, J
dc.contributor.authorGoyal, P
dc.contributor.authorClaesson, E
dc.contributor.authorCaing-Carlsson, R
dc.contributor.authorAndersson, R
dc.contributor.authorBeis, K
dc.contributor.authorNilsson, UJ
dc.contributor.authorFarewell, A
dc.contributor.authorPochini, L
dc.contributor.authorIndiveri, C
dc.contributor.authorGrabe, M
dc.contributor.authorDobson, RCJ
dc.contributor.authorAbramson, J
dc.contributor.authorRamaswamy, S
dc.contributor.authorFriemann, R
dc.date.accessioned2020-12-17T03:42:42Z
dc.date.available2020-12-17T03:42:42Z
dc.date.issued2018-05-01
dc.identifierpii: 10.1038/s41467-018-04045-7
dc.identifier.citationWahlgren, W. Y., Dunevall, E., North, R. A., Paz, A., Scalise, M., Bisignano, P., Bengtsson-Palme, J., Goyal, P., Claesson, E., Caing-Carlsson, R., Andersson, R., Beis, K., Nilsson, U. J., Farewell, A., Pochini, L., Indiveri, C., Grabe, M., Dobson, R. C. J., Abramson, J. ,... Friemann, R. (2018). Substrate-bound outward-open structure of a Na+-coupled sialic acid symporter reveals a new Na+ site. NATURE COMMUNICATIONS, 9 (1), https://doi.org/10.1038/s41467-018-04045-7.
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/11343/254999
dc.description.abstractMany pathogenic bacteria utilise sialic acids as an energy source or use them as an external coating to evade immune detection. As such, bacteria that colonise sialylated environments deploy specific transporters to mediate import of scavenged sialic acids. Here, we report a substrate-bound 1.95 Å resolution structure and subsequent characterisation of SiaT, a sialic acid transporter from Proteus mirabilis. SiaT is a secondary active transporter of the sodium solute symporter (SSS) family, which use Na+ gradients to drive the uptake of extracellular substrates. SiaT adopts the LeuT-fold and is in an outward-open conformation in complex with the sialic acid N-acetylneuraminic acid and two Na+ ions. One Na+ binds to the conserved Na2 site, while the second Na+ binds to a new position, termed Na3, which is conserved in many SSS family members. Functional and molecular dynamics studies validate the substrate-binding site and demonstrate that both Na+ sites regulate N-acetylneuraminic acid transport.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleSubstrate-bound outward-open structure of a Na+-coupled sialic acid symporter reveals a new Na+ site
dc.typeJournal Article
dc.identifier.doi10.1038/s41467-018-04045-7
melbourne.affiliation.departmentBiochemistry and Molecular Biology
melbourne.source.titleNature Communications
melbourne.source.volume9
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1328718
melbourne.contributor.authorDobson, Renwick
dc.identifier.eissn2041-1723
melbourne.accessrightsOpen Access


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