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    CLASP1 regulates endothelial cell branching morphology and directed migration

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    Author
    Myer, NM; Myers, KA
    Date
    2017-10-15
    Source Title
    Biology Open
    Publisher
    COMPANY BIOLOGISTS LTD
    University of Melbourne Author/s
    Myers, Kenneth
    Affiliation
    Medicine and Radiology
    Metadata
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    Document Type
    Journal Article
    Citations
    Myer, N. M. & Myers, K. A. (2017). CLASP1 regulates endothelial cell branching morphology and directed migration. BIOLOGY OPEN, 6 (10), pp.1502-1515. https://doi.org/10.1242/bio.028571.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/255055
    DOI
    10.1242/bio.028571
    Abstract
    Endothelial cell (EC) branching is critically dependent upon the dynamic nature of the microtubule (MT) cytoskeleton. Extracellular matrix (ECM) mechanosensing is a prominent mechanism by which cytoskeletal reorganization is achieved; yet how ECM-induced signaling is able to target cytoskeletal reorganization intracellularly to facilitate productive EC branching morphogenesis is not known. Here, we tested the hypothesis that the composition and density of the ECM drive the regulation of MT growth dynamics in ECs by targeting the MT stabilizing protein, cytoplasmic linker associated protein 1 (CLASP1). High-resolution fluorescent microscopy coupled with computational image analysis reveal that CLASP1 promotes slow MT growth on glass ECMs and promotes short-lived MT growth on high-density collagen-I and fibronectin ECMs. Within EC branches, engagement of either high-density collagen-I or high-density fibronectin ECMs results in reduced MT growth speeds, while CLASP1-dependent effects on MT dynamics promotes elevated numbers of short, branched protrusions that guide persistent and directed EC migration.

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