CLASP1 regulates endothelial cell branching morphology and directed migration

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Myer, NM; Myers, KADate
2017-10-15Source Title
Biology OpenPublisher
COMPANY BIOLOGISTS LTDUniversity of Melbourne Author/s
Myers, KennethAffiliation
Medicine and RadiologyMetadata
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Journal ArticleCitations
Myer, N. M. & Myers, K. A. (2017). CLASP1 regulates endothelial cell branching morphology and directed migration. BIOLOGY OPEN, 6 (10), pp.1502-1515. https://doi.org/10.1242/bio.028571.Access Status
Open AccessAbstract
Endothelial cell (EC) branching is critically dependent upon the dynamic nature of the microtubule (MT) cytoskeleton. Extracellular matrix (ECM) mechanosensing is a prominent mechanism by which cytoskeletal reorganization is achieved; yet how ECM-induced signaling is able to target cytoskeletal reorganization intracellularly to facilitate productive EC branching morphogenesis is not known. Here, we tested the hypothesis that the composition and density of the ECM drive the regulation of MT growth dynamics in ECs by targeting the MT stabilizing protein, cytoplasmic linker associated protein 1 (CLASP1). High-resolution fluorescent microscopy coupled with computational image analysis reveal that CLASP1 promotes slow MT growth on glass ECMs and promotes short-lived MT growth on high-density collagen-I and fibronectin ECMs. Within EC branches, engagement of either high-density collagen-I or high-density fibronectin ECMs results in reduced MT growth speeds, while CLASP1-dependent effects on MT dynamics promotes elevated numbers of short, branched protrusions that guide persistent and directed EC migration.
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