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dc.contributor.authorClark, NM
dc.contributor.authorHinde, E
dc.contributor.authorWinter, CM
dc.contributor.authorFisher, AP
dc.contributor.authorCrosti, G
dc.contributor.authorBlilou, I
dc.contributor.authorGratton, E
dc.contributor.authorBenfey, PN
dc.contributor.authorSozzani, R
dc.date.accessioned2020-12-17T03:54:48Z
dc.date.available2020-12-17T03:54:48Z
dc.date.issued2016-06-11
dc.identifier.citationClark, N. M., Hinde, E., Winter, C. M., Fisher, A. P., Crosti, G., Blilou, I., Gratton, E., Benfey, P. N. & Sozzani, R. (2016). Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy.. Elife, 5 (JUN2016), pp.e14770-. https://doi.org/10.7554/eLife.14770.
dc.identifier.issn2050-084X
dc.identifier.urihttp://hdl.handle.net/11343/255085
dc.description.abstractTo understand complex regulatory processes in multicellular organisms, it is critical to be able to quantitatively analyze protein movement and protein-protein interactions in time and space. During Arabidopsis development, the intercellular movement of SHORTROOT (SHR) and subsequent interaction with its downstream target SCARECROW (SCR) control root patterning and cell fate specification. However, quantitative information about the spatio-temporal dynamics of SHR movement and SHR-SCR interaction is currently unavailable. Here, we quantify parameters including SHR mobility, oligomeric state, and association with SCR using a combination of Fluorescent Correlation Spectroscopy (FCS) techniques. We then incorporate these parameters into a mathematical model of SHR and SCR, which shows that SHR reaches a steady state in minutes, while SCR and the SHR-SCR complex reach a steady-state between 18 and 24 hr. Our model reveals the timing of SHR and SCR dynamics and allows us to understand how protein movement and protein-protein stoichiometry contribute to development.
dc.languageeng
dc.publishereLife Sciences Publications, Ltd
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleTracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy.
dc.typeJournal Article
dc.identifier.doi10.7554/eLife.14770
melbourne.affiliation.departmentSchool of Physics
melbourne.source.titleeLife
melbourne.source.volume5
melbourne.source.issueJUN2016
melbourne.source.pagese14770-
dc.rights.licenseCC BY
melbourne.elementsid1234116
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946880
melbourne.contributor.authorHinde, Elizabeth
dc.identifier.eissn2050-084X
melbourne.accessrightsOpen Access


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