University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Medical Biology
  • Medical Biology - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Medical Biology
  • Medical Biology - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Cre transgene results in global attenuation of the cAMP/PKA pathway.

    Thumbnail
    Download
    Published version (403.9Kb)

    Citations
    Scopus
    Web of Science
    Altmetric
    11
    13
    Author
    Gangoda, L; Doerflinger, M; Lee, YY; Rahimi, A; Etemadi, N; Chau, D; Milla, L; O'Connor, L; Puthalakath, H
    Date
    2012-08-09
    Source Title
    Cell Death and Disease
    Publisher
    Springer Science and Business Media LLC
    University of Melbourne Author/s
    Doerflinger, Marcel
    Affiliation
    Medical Biology (W.E.H.I.)
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Gangoda, L., Doerflinger, M., Lee, Y. Y., Rahimi, A., Etemadi, N., Chau, D., Milla, L., O'Connor, L. & Puthalakath, H. (2012). Cre transgene results in global attenuation of the cAMP/PKA pathway.. Cell Death Dis, 3 (8), pp.e365-. https://doi.org/10.1038/cddis.2012.110.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/255124
    DOI
    10.1038/cddis.2012.110
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434654
    Abstract
    Use of the cre transgene in in vivo mouse models to delete a specific 'floxed' allele is a well-accepted method for studying the effects of spatially or temporarily regulated genes. During the course of our investigation into the effect of cyclic adenosine 3',5'-monophosphate-dependent protein kinase A (PKA) expression on cell death, we found that cre expression either in cultured cell lines or in transgenic mice results in global changes in PKA target phosphorylation. This consequently alters gene expression profile and changes in cytokine secretion such as IL-6. These effects are dependent on its recombinase activity and can be attributed to the upregulation of specific inhibitors of PKA (PKI). These results may explain the cytotoxicity often associated with cre expression in many transgenic animals and may also explain many of the phenotypes observed in the context of Cre-mediated gene deletion. Our results may therefore influence the interpretation of data generated using the conventional cre transgenic system.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [45770]
    • Medical Biology - Research Publications [865]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors