Show simple item record

dc.contributor.authorGangoda, L
dc.contributor.authorDoerflinger, M
dc.contributor.authorLee, YY
dc.contributor.authorRahimi, A
dc.contributor.authorEtemadi, N
dc.contributor.authorChau, D
dc.contributor.authorMilla, L
dc.contributor.authorO'Connor, L
dc.contributor.authorPuthalakath, H
dc.date.accessioned2020-12-17T04:00:03Z
dc.date.available2020-12-17T04:00:03Z
dc.date.issued2012-08-09
dc.identifierpii: cddis2012110
dc.identifier.citationGangoda, L., Doerflinger, M., Lee, Y. Y., Rahimi, A., Etemadi, N., Chau, D., Milla, L., O'Connor, L. & Puthalakath, H. (2012). Cre transgene results in global attenuation of the cAMP/PKA pathway.. Cell Death Dis, 3 (8), pp.e365-. https://doi.org/10.1038/cddis.2012.110.
dc.identifier.issn2041-4889
dc.identifier.urihttp://hdl.handle.net/11343/255124
dc.description.abstractUse of the cre transgene in in vivo mouse models to delete a specific 'floxed' allele is a well-accepted method for studying the effects of spatially or temporarily regulated genes. During the course of our investigation into the effect of cyclic adenosine 3',5'-monophosphate-dependent protein kinase A (PKA) expression on cell death, we found that cre expression either in cultured cell lines or in transgenic mice results in global changes in PKA target phosphorylation. This consequently alters gene expression profile and changes in cytokine secretion such as IL-6. These effects are dependent on its recombinase activity and can be attributed to the upregulation of specific inhibitors of PKA (PKI). These results may explain the cytotoxicity often associated with cre expression in many transgenic animals and may also explain many of the phenotypes observed in the context of Cre-mediated gene deletion. Our results may therefore influence the interpretation of data generated using the conventional cre transgenic system.
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.titleCre transgene results in global attenuation of the cAMP/PKA pathway.
dc.typeJournal Article
dc.identifier.doi10.1038/cddis.2012.110
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleCell Death and Disease
melbourne.source.volume3
melbourne.source.issue8
melbourne.source.pagese365-
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1238047
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434654
melbourne.contributor.authorDoerflinger, Marcel
dc.identifier.eissn2041-4889
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record