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dc.contributor.authorEbert, B
dc.contributor.authorZoeller, D
dc.contributor.authorErban, A
dc.contributor.authorFehrle, I
dc.contributor.authorHartmann, J
dc.contributor.authorNiehl, A
dc.contributor.authorKopka, J
dc.contributor.authorFisahn, J
dc.date.accessioned2020-12-17T04:02:12Z
dc.date.available2020-12-17T04:02:12Z
dc.date.issued2010-03-01
dc.identifierpii: erq002
dc.identifier.citationEbert, B., Zoeller, D., Erban, A., Fehrle, I., Hartmann, J., Niehl, A., Kopka, J. & Fisahn, J. (2010). Metabolic profiling of Arabidopsis thaliana epidermal cells. JOURNAL OF EXPERIMENTAL BOTANY, 61 (5), pp.1321-1335. https://doi.org/10.1093/jxb/erq002.
dc.identifier.issn0022-0957
dc.identifier.urihttp://hdl.handle.net/11343/255134
dc.description.abstractMetabolic phenotyping at cellular resolution may be considered one of the challenges in current plant physiology. A method is described which enables the cell type-specific metabolic analysis of epidermal cell types in Arabidopsis thaliana pavement, basal, and trichome cells. To achieve the required high spatial resolution, single cell sampling using microcapillaries was combined with routine gas chromatography-time of flight-mass spectrometry (GC-TOF-MS) based metabolite profiling. The identification and relative quantification of 117 mostly primary metabolites has been demonstrated. The majority, namely 90 compounds, were accessible without analytical background correction. Analyses were performed using cell type-specific pools of 200 microsampled individual cells. Moreover, among these identified metabolites, 38 exhibited differential pool sizes in trichomes, basal or pavement cells. The application of an independent component analysis confirmed the cell type-specific metabolic phenotypes. Significant pool size changes between individual cells were detectable within several classes of metabolites, namely amino acids, fatty acids and alcohols, alkanes, lipids, N-compounds, organic acids and polyhydroxy acids, polyols, sugars, sugar conjugates and phenylpropanoids. It is demonstrated here that the combination of microsampling and GC-MS based metabolite profiling provides a method to investigate the cellular metabolism of fully differentiated plant cell types in vivo.
dc.languageEnglish
dc.publisherOXFORD UNIV PRESS
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0
dc.titleMetabolic profiling of Arabidopsis thaliana epidermal cells
dc.typeJournal Article
dc.identifier.doi10.1093/jxb/erq002
melbourne.affiliation.departmentSchool of BioSciences
melbourne.source.titleJournal of Experimental Botany
melbourne.source.volume61
melbourne.source.issue5
melbourne.source.pages1321-1335
dc.rights.licenseCC BY-NC
melbourne.elementsid1330135
melbourne.contributor.authorEbert, Berit
dc.identifier.eissn1460-2431
melbourne.accessrightsOpen Access


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