University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Microbiology & Immunology
  • Microbiology & Immunology - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Microbiology & Immunology
  • Microbiology & Immunology - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    The First Isolation and Whole Genome Sequencing of Murray Valley Encephalitis Virus from Cerebrospinal Fluid of a Patient with Encephalitis

    Thumbnail
    Download
    Published version (514.3Kb)

    Citations
    Scopus
    Web of Science
    Altmetric
    4
    4
    Author
    Russell, JS; Caly, L; Kostecki, R; McGuinness, SL; Carter, G; Bulach, D; Seemann, T; Stinear, TP; Baird, R; Catton, M; ...
    Date
    2018-06-01
    Source Title
    Viruses
    Publisher
    MDPI
    University of Melbourne Author/s
    Seemann, Torsten; Carter, Glen; Stinear, Timothy; Caly, Leon; Bulach, Dieter
    Affiliation
    Microbiology and Immunology
    Doherty Institute
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Russell, J. S., Caly, L., Kostecki, R., McGuinness, S. L., Carter, G., Bulach, D., Seemann, T., Stinear, T. P., Baird, R., Catton, M. & Druce, J. (2018). The First Isolation and Whole Genome Sequencing of Murray Valley Encephalitis Virus from Cerebrospinal Fluid of a Patient with Encephalitis. VIRUSES-BASEL, 10 (6), https://doi.org/10.3390/v10060319.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/255205
    DOI
    10.3390/v10060319
    Abstract
    Murray Valley Encephalitis virus (MVEV) is a mosquito-borne Flavivirus. Clinical presentation is rare but severe, with a case fatality rate of 15⁻30%. Here we report a case of MVEV from the cerebrospinal fluid (CSF) of a patient in the Northern Territory in Australia. Initial diagnosis was performed using both MVEV-specific real-time, and Pan-Flavivirus conventional, Polymerase Chain Reaction (PCR), with confirmation by Sanger sequencing. Subsequent isolation, the first from CSF, was conducted in Vero cells and the observed cytopathic effect was confirmed by increasing viral titre in the real-time PCR. Isolation allowed for full genome sequencing using the Scriptseq V2 RNASeq library preparation kit. A consensus genome for VIDRL-MVE was generated and phylogenetic analysis identified it as Genotype 2. This is the first reported isolation, and full genome sequencing of MVEV from CSF. It is also the first time Genotype 2 has been identified in humans. As such, this case has significant implications for public health surveillance, epidemiology, and the understanding of MVEV evolution.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [52443]
    • Doherty Institute - Research Publications [373]
    • Microbiology & Immunology - Research Publications [2198]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors