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dc.contributor.authorCarew, ME
dc.contributor.authorColeman, RA
dc.contributor.authorHoffman, AA
dc.date.accessioned2020-12-17T04:34:05Z
dc.date.available2020-12-17T04:34:05Z
dc.date.issued2018-06-13
dc.identifierpii: 4980
dc.identifier.citationCarew, M. E., Coleman, R. A. & Hoffman, A. A. (2018). Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?. PEERJ, 6 (6), https://doi.org/10.7717/peerj.4980.
dc.identifier.issn2167-8359
dc.identifier.urihttp://hdl.handle.net/11343/255364
dc.description.abstractBackground: High throughput DNA sequencing of bulk invertebrate samples or metabarcoding is becoming increasingly used to provide profiles of biological communities for environmental monitoring. As metabarcoding becomes more widely applied, new reference DNA barcodes linked to individual specimens identified by taxonomists are needed. This can be achieved through using DNA extraction methods that are not only suitable for metabarcoding but also for building reference DNA barcode libraries. Methods: In this study, we test the suitability of a rapid non-destructive DNA extraction method for metabarcoding of freshwater invertebrate samples. Results: This method resulted in detection of taxa from many taxonomic groups, comparable to results obtained with two other tissue-based extraction methods. Most taxa could also be successfully used for subsequent individual-based DNA barcoding and taxonomic identification. The method was successfully applied to field-collected invertebrate samples stored for taxonomic studies in 70% ethanol at room temperature, a commonly used storage method for freshwater samples. Discussion: With further refinement and testing, non-destructive extraction has the potential to rapidly characterise species biodiversity in invertebrate samples, while preserving specimens for taxonomic investigation.
dc.languageEnglish
dc.publisherPEERJ INC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleCan non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?
dc.typeJournal Article
dc.identifier.doi10.7717/peerj.4980
melbourne.affiliation.departmentSchool of BioSciences
melbourne.source.titlePeerJ
melbourne.source.volume6
melbourne.source.issue6
melbourne.identifier.arcFL100100066
melbourne.identifier.arcLP150100876
dc.rights.licenseCC BY
melbourne.elementsid1336343
melbourne.contributor.authorHoffmann, Ary
melbourne.contributor.authorCarew, Melissa
dc.identifier.eissn2167-8359
melbourne.identifier.fundernameidAUST RESEARCH COUNCIL, FL100100066
melbourne.identifier.fundernameidAustralian Research Council, LP150100876
melbourne.accessrightsOpen Access


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