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    The effects of knee injury on skeletal muscle function, Na+, K+-ATPase content, and isoform abundance

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    12
    Author
    Perry, BD; Levinger, P; Morris, HG; Petersen, AC; Garnham, AP; Levinger, I; McKenna, MJ
    Date
    2015-02-01
    Source Title
    Physiological Reports
    Publisher
    WILEY
    University of Melbourne Author/s
    Morris, Hayden; Levinger, Itamar
    Affiliation
    Medical Education
    Medicine and Radiology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Perry, B. D., Levinger, P., Morris, H. G., Petersen, A. C., Garnham, A. P., Levinger, I. & McKenna, M. J. (2015). The effects of knee injury on skeletal muscle function, Na+, K+-ATPase content, and isoform abundance. PHYSIOLOGICAL REPORTS, 3 (2), https://doi.org/10.14814/phy2.12294.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/255405
    DOI
    10.14814/phy2.12294
    Abstract
    While training upregulates skeletal muscle Na(+), K(+)-ATPase (NKA), the effects of knee injury and associated disuse on muscle NKA remain unknown. This was therefore investigated in six healthy young adults with a torn anterior cruciate ligament, (KI; four females, two males; age 25.0 ± 4.9 years; injury duration 15 ± 17 weeks; mean ± SD) and seven age- and BMI-matched asymptomatic controls (CON; five females, two males). Each participant underwent a vastus lateralis muscle biopsy, on both legs in KI and one leg in CON. Muscle was analyzed for muscle fiber type and cross-sectional area (CSA), NKA content ([(3)H]ouabain binding), and α1-3 and β1-2 isoform abundance. Participants also completed physical activity and knee function questionnaires (KI only); and underwent quadriceps peak isometric strength, thigh CSA and postural sway assessments in both injured and noninjured legs. NKA content was 20.1% lower in the knee-injured leg than the noninjured leg and 22.5% lower than CON. NKA α2 abundance was 63.0% lower in the knee-injured leg than the noninjured leg, with no differences in other NKA isoforms. Isometric strength and thigh CSA were 21.7% and 7.1% lower in the injured leg than the noninjured leg, respectively. In KI, postural sway did not differ between legs, but for two-legged standing was 43% higher than CON. Hence, muscle NKA content and α2 abundance were reduced in severe knee injury, which may contribute to impaired muscle function. Restoration of muscle NKA may be important in rehabilitation of muscle function after knee and other lower limb injury.

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