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dc.contributor.authorJud, C
dc.contributor.authorAhmed, S
dc.contributor.authorMueller, L
dc.contributor.authorKinnear, C
dc.contributor.authorVanhecke, D
dc.contributor.authorUmehara, Y
dc.contributor.authorFrey, S
dc.contributor.authorLiley, M
dc.contributor.authorAngeloni, S
dc.contributor.authorPetri-Fink, A
dc.contributor.authorRothen-Rutishauser, B
dc.date.accessioned2020-12-18T02:39:24Z
dc.date.available2020-12-18T02:39:24Z
dc.date.issued2015-12-01
dc.identifierpii: 10.1089/biores.2015.0037
dc.identifier.citationJud, C., Ahmed, S., Mueller, L., Kinnear, C., Vanhecke, D., Umehara, Y., Frey, S., Liley, M., Angeloni, S., Petri-Fink, A. & Rothen-Rutishauser, B. (2015). Ultrathin Ceramic Membranes as Scaffolds for Functional Cell Coculture Models on a Biomimetic Scale. BIORESEARCH OPEN ACCESS, 4 (1), pp.457-468. https://doi.org/10.1089/biores.2015.0037.
dc.identifier.issn2164-7860
dc.identifier.urihttp://hdl.handle.net/11343/255440
dc.description.abstractEpithelial tissue serves as an interface between biological compartments. Many in vitro epithelial cell models have been developed as an alternative to animal experiments to answer a range of research questions. These in vitro models are grown on permeable two-chamber systems; however, commercially available, polymer-based cell culture inserts are around 10 μm thick. Since the basement membrane found in biological systems is usually less than 1 μm thick, the 10-fold thickness of cell culture inserts is a major limitation in the establishment of realistic models. In this work, an alternative insert, accommodating an ultrathin ceramic membrane with a thickness of only 500 nm (i.e., the Silicon nitride Microporous Permeable Insert [SIMPLI]-well), was produced and used to refine an established human alveolar barrier coculture model by both replacing the conventional inserts with the SIMPLI-well and completing it with endothelial cells. The structural-functional relationship of the model was evaluated, including the translocation of gold nanoparticles across the barrier, revealing a higher translocation if compared to corresponding polyethylene terephthalate (PET) membranes. This study demonstrates the power of the SIMPLI-well system as a scaffold for epithelial tissue cell models on a truly biomimetic scale, allowing construction of more functionally accurate models of human biological barriers.
dc.languageEnglish
dc.publisherMARY ANN LIEBERT, INC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleUltrathin Ceramic Membranes as Scaffolds for Functional Cell Coculture Models on a Biomimetic Scale
dc.typeJournal Article
dc.identifier.doi10.1089/biores.2015.0037
melbourne.affiliation.departmentSchool of Chemistry
melbourne.source.titleBioResearch Open Access
melbourne.source.volume4
melbourne.source.issue1
melbourne.source.pages457-468
dc.rights.licenseCC BY
melbourne.elementsid1279834
melbourne.contributor.authorKinnear, Calum
dc.identifier.eissn2164-7860
melbourne.accessrightsOpen Access


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