University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Science
  • School of Physics
  • School of Physics - Research Publications
  • View Item
  • Minerva Access
  • Science
  • School of Physics
  • School of Physics - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    An organic fluorophorenanodiamond hybrid sensor for photostable imaging and orthogonal, on-demand biosensing

    Thumbnail
    Download
    Published version (1.969Mb)

    Citations
    Scopus
    Web of Science
    Altmetric
    5
    5
    Author
    Purdey, MS; Capon, PK; Pullen, BJ; Reineck, P; Schwarz, N; Psaltis, PJ; Nicholls, SJ; Gibson, BC; Abell, AD
    Date
    2017-11-21
    Source Title
    Scientific Reports
    Publisher
    NATURE PUBLISHING GROUP
    University of Melbourne Author/s
    Gibson, Brant
    Affiliation
    School of Physics
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Purdey, M. S., Capon, P. K., Pullen, B. J., Reineck, P., Schwarz, N., Psaltis, P. J., Nicholls, S. J., Gibson, B. C. & Abell, A. D. (2017). An organic fluorophorenanodiamond hybrid sensor for photostable imaging and orthogonal, on-demand biosensing. SCIENTIFIC REPORTS, 7 (1), https://doi.org/10.1038/s41598-017-15772-0.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/255441
    DOI
    10.1038/s41598-017-15772-0
    Abstract
    Organic fluorescent probes are widely used to detect key biomolecules; however, they often lack the photostability required for extended intracellular imaging. Here we report a new hybrid nanomaterial (peroxynanosensor, PNS), consisting of an organic fluorescent probe bound to a nanodiamond, that overcomes this limitation to allow concurrent and extended cell-based imaging of the nanodiamond and ratiometric detection of hydrogen peroxide. Far-red fluorescence of the nanodiamond offers continuous monitoring without photobleaching, while the green fluorescence of the organic fluorescent probe attached to the nanodiamond surface detects hydrogen peroxide on demand. PNS detects basal production of hydrogen peroxide within M1 polarised macrophages and does not affect macrophage growth during prolonged co-incubation. This nanosensor can be used for extended bio-imaging not previously possible with an organic fluorescent probe, and is spectrally compatible with both Hoechst 33342 and MitoTracker Orange stains for hyperspectral imaging.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [53102]
    • School of Physics - Research Publications [1059]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors