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dc.contributor.authorAlsanie, WF
dc.contributor.authorNiclis, JC
dc.contributor.authorHunt, CP
dc.contributor.authorDe Luzy, IR
dc.contributor.authorPenna, V
dc.contributor.authorBye, CR
dc.contributor.authorPouton, CW
dc.contributor.authorHaynes, J
dc.contributor.authorFiras, J
dc.contributor.authorThompson, LH
dc.contributor.authorParish, CL
dc.date.accessioned2020-12-18T02:42:53Z
dc.date.available2020-12-18T02:42:53Z
dc.date.issued2017-11-22
dc.identifierpii: 10.1038/s41598-017-16248-x
dc.identifier.citationAlsanie, W. F., Niclis, J. C., Hunt, C. P., De Luzy, I. R., Penna, V., Bye, C. R., Pouton, C. W., Haynes, J., Firas, J., Thompson, L. H. & Parish, C. L. (2017). Specification of murine ground state pluripotent stem cells to regional neuronal populations. SCIENTIFIC REPORTS, 7 (1), https://doi.org/10.1038/s41598-017-16248-x.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/255466
dc.description.abstractPluripotent stem cells (PSCs) are a valuable tool for interrogating development, disease modelling, drug discovery and transplantation. Despite the burgeoned capability to fate restrict human PSCs to specific neural lineages, comparative protocols for mouse PSCs have not similarly advanced. Mouse protocols fail to recapitulate neural development, consequently yielding highly heterogeneous populations, yet mouse PSCs remain a valuable scientific tool as differentiation is rapid, cost effective and an extensive repertoire of transgenic lines provides an invaluable resource for understanding biology. Here we developed protocols for neural fate restriction of mouse PSCs, using knowledge of embryonic development and recent progress with human equivalents. These methodologies rely upon naïve ground-state PSCs temporarily transitioning through LIF-responsive stage prior to neural induction and rapid exposure to regional morphogens. Neural subtypes generated included those of the dorsal forebrain, ventral forebrain, ventral midbrain and hindbrain. This rapid specification, without feeder layers or embryoid-body formation, resulted in high proportions of correctly specified progenitors and neurons with robust reproducibility. These generated neural progenitors/neurons will provide a valuable resource to further understand development, as well disorders affecting specific neuronal subpopulations.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleSpecification of murine ground state pluripotent stem cells to regional neuronal populations
dc.typeJournal Article
dc.identifier.doi10.1038/s41598-017-16248-x
melbourne.affiliation.departmentFlorey Department of Neuroscience and Mental Health
melbourne.affiliation.departmentAnatomy and Neuroscience
melbourne.source.titleScientific Reports
melbourne.source.volume7
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1282140
melbourne.contributor.authorde Luzy, Isabelle
melbourne.contributor.authorParish, Clare
melbourne.contributor.authorAlsanie, Walaa
melbourne.contributor.authorHunt, Cameron
melbourne.contributor.authorPenna, Vanessa
melbourne.contributor.authorNICLIS, JONATHAN
melbourne.contributor.authorBye, Chris
melbourne.contributor.authorPOUTON, COLIN
melbourne.contributor.authorThompson, Lachlan
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


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