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dc.contributor.authorLongley, RJ
dc.contributor.authorHalbroth, BR
dc.contributor.authorSalman, AM
dc.contributor.authorEwer, KJ
dc.contributor.authorHodgson, SH
dc.contributor.authorJanse, CJ
dc.contributor.authorKhan, SM
dc.contributor.authorHill, AVS
dc.contributor.authorSpencer, AJ
dc.date.accessioned2020-12-18T02:51:41Z
dc.date.available2020-12-18T02:51:41Z
dc.date.issued2017-03-01
dc.identifierpii: IAI.00641-16
dc.identifier.citationLongley, R. J., Halbroth, B. R., Salman, A. M., Ewer, K. J., Hodgson, S. H., Janse, C. J., Khan, S. M., Hill, A. V. S. & Spencer, A. J. (2017). Assessment of the Plasmodium falciparum Preerythrocytic Antigen UIS3 as a Potential Candidate for a Malaria Vaccine. INFECTION AND IMMUNITY, 85 (3), https://doi.org/10.1128/IAI.00641-16.
dc.identifier.issn0019-9567
dc.identifier.urihttp://hdl.handle.net/11343/255528
dc.description.abstractEfforts are under way to improve the efficacy of subunit malaria vaccines through assessments of new adjuvants, vaccination platforms, and antigens. In this study, we further assessed the Plasmodium falciparum antigen upregulated in infective sporozoites 3 (PfUIS3) as a vaccine candidate. PfUIS3 was expressed in the viral vectors chimpanzee adenovirus 63 (ChAd63) and modified vaccinia virus Ankara (MVA) and used to immunize mice in a prime-boost regimen. We previously demonstrated that this regimen could provide partial protection against challenge with chimeric P. berghei parasites expressing PfUIS3. We now show that ChAd63-MVA PfUIS3 can also provide partial cross-species protection against challenge with wild-type P. berghei parasites. We also show that PfUIS3-specific cellular memory responses could be recalled in human volunteers exposed to P. falciparum parasites in a controlled human malaria infection study. When ChAd63-MVA PfUIS3 was coadministered with the vaccine candidate P. falciparum thrombospondin-related adhesion protein (PfTRAP) expressed in the ChAd63-MVA system, there was no significant change in immunogenicity to either vaccine. However, when mice were challenged with double chimeric P. berghei-P. falciparum parasites expressing both PfUIS3 and PfTRAP, vaccine efficacy was improved to 100% sterile protection. This synergistic effect was evident only when the two vaccines were mixed and administered at the same site. We have therefore demonstrated that vaccination with PfUIS3 can induce a consistent delay in patent parasitemia across mouse strains and against chimeric parasites expressing PfUIS3 as well as wild-type P. berghei; when this vaccine is combined with another partially protective regimen (ChAd63-MVA PfTRAP), complete protection is induced.
dc.languageEnglish
dc.publisherAMER SOC MICROBIOLOGY
dc.titleAssessment of the Plasmodium falciparum Preerythrocytic Antigen UIS3 as a Potential Candidate for a Malaria Vaccine
dc.typeJournal Article
dc.identifier.doi10.1128/IAI.00641-16
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleInfection and Immunity
melbourne.source.volume85
melbourne.source.issue3
dc.rights.licenseCC BY
melbourne.elementsid1190955
melbourne.contributor.authorLongley, Rhea
dc.identifier.eissn1098-5522
melbourne.accessrightsOpen Access


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