University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Affiliates
  • Bio21
  • Bio21 - Research Publications
  • View Item
  • Minerva Access
  • Affiliates
  • Bio21
  • Bio21 - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Visualising single molecules of HIV-1 and miRNA nucleic acids

    Thumbnail
    Download
    Published version (3.667Mb)

    Citations
    Scopus
    Web of Science
    Altmetric
    2
    2
    Author
    Jones, KL; Karpala, A; Hirst, B; Jenkins, K; Tizard, M; Pereira, CF; Leis, A; Monaghan, P; Hyatt, A; Mak, J
    Date
    2013-04-17
    Source Title
    BMC Cell Biology
    Publisher
    BMC
    University of Melbourne Author/s
    Leis, Andrew
    Affiliation
    Bio21
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Jones, K. L., Karpala, A., Hirst, B., Jenkins, K., Tizard, M., Pereira, C. F., Leis, A., Monaghan, P., Hyatt, A. & Mak, J. (2013). Visualising single molecules of HIV-1 and miRNA nucleic acids. BMC CELL BIOLOGY, 14 (1), https://doi.org/10.1186/1471-2121-14-21.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/255547
    DOI
    10.1186/1471-2121-14-21
    Abstract
    BACKGROUND: The scarcity of certain nucleic acid species and the small size of target sequences such as miRNA, impose a significant barrier to subcellular visualization and present a major challenge to cell biologists. Here, we offer a generic and highly sensitive visualization approach (oligo fluorescent in situ hybridization, O-FISH) that can be used to detect such nucleic acids using a single-oligonucleotide probe of 19-26 nucleotides in length. RESULTS: We used O-FISH to visualize miR146a in human and avian cells. Furthermore, we reveal the sensitivity of O-FISH detection by using a HIV-1 model system to show that as little as 1-2 copies of nucleic acids can be detected in a single cell. We were able to discern newly synthesized viral cDNA and, moreover, observed that certain HIV RNA sequences are only transiently available for O-FISH detection. CONCLUSIONS: Taken together, these results suggest that the O-FISH method can potentially be used for in situ probing of, as few as, 1-2 copies of nucleic acid and, additionally, to visualize small RNA such as miRNA. We further propose that the O-FISH method could be extended to understand viral function by probing newly transcribed viral intermediates; and discern the localisation of nucleic acids of interest. Additionally, interrogating the conformation and structure of a particular nucleic acid in situ might also be possible, based on the accessibility of a target sequence.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [45770]
    • Bio21 - Research Publications [232]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors