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dc.contributor.authorZhao, X
dc.contributor.authorLiu, N
dc.contributor.authorShang, N
dc.contributor.authorZeng, W
dc.contributor.authorEbert, B
dc.contributor.authorRautengarten, C
dc.contributor.authorZeng, Q-Y
dc.contributor.authorLi, H
dc.contributor.authorChen, X
dc.contributor.authorBeahan, C
dc.contributor.authorBacic, A
dc.contributor.authorHeazlewood, JL
dc.contributor.authorWu, A-M
dc.date.accessioned2020-12-18T02:58:37Z
dc.date.available2020-12-18T02:58:37Z
dc.date.issued2018-02-20
dc.identifierpii: 4781077
dc.identifier.citationZhao, X., Liu, N., Shang, N., Zeng, W., Ebert, B., Rautengarten, C., Zeng, Q. -Y., Li, H., Chen, X., Beahan, C., Bacic, A., Heazlewood, J. L. & Wu, A. -M. (2018). Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis. JOURNAL OF EXPERIMENTAL BOTANY, 69 (5), pp.1125-1134. https://doi.org/10.1093/jxb/erx448.
dc.identifier.issn0022-0957
dc.identifier.urihttp://hdl.handle.net/11343/255581
dc.description.abstractUDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs). The Arabidopsis genome encodes six UXSs, which fall into two groups based upon their subcellular location: the Golgi lumen and the cytosol. The latter group appears to play an important role in xylan biosynthesis. Cytosolic UDP-Xyl is transported into the Golgi lumen by three UDP-Xyl transporters (UXT1, 2, and 3). However, while single mutants affected in the UDP-Xyl transporter 1 (UXT1) showed a substantial reduction in cell wall xylose content, a double mutant affected in UXT2 and UXT3 had no obvious effect on cell wall xylose deposition. This prompted us to further investigate redundancy among the members of the UXT family. Multiple uxt mutants were generated, including a triple mutant, which exhibited collapsed vessels and reduced cell wall thickness in interfascicular fiber cells. Monosaccharide composition, molecular weight, nuclear magnetic resonance, and immunolabeling studies demonstrated that both xylan biosynthesis (content) and fine structure were significantly affected in the uxt triple mutant, leading to phenotypes resembling those of the irx mutants. Pollination was also impaired in the uxt triple mutant, likely due to reduced filament growth and anther dehiscence caused by alterations in the composition of the cell walls. Moreover, analysis of the nucleotide sugar composition of the uxt mutants indicated that nucleotide sugar interconversion is influenced by the cytosolic UDP-Xyl pool within the cell. Taken together, our results underpin the physiological roles of the UXT family in xylan biosynthesis and provide novel insights into the nucleotide sugar metabolism and trafficking in plants.
dc.languageEnglish
dc.publisherOXFORD UNIV PRESS
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleThree UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis
dc.typeJournal Article
dc.identifier.doi10.1093/jxb/erx448
melbourne.affiliation.departmentSchool of BioSciences
melbourne.source.titleJournal of Experimental Botany
melbourne.source.volume69
melbourne.source.issue5
melbourne.source.pages1125-1134
melbourne.identifier.arcFT130101165
melbourne.identifier.arcFT160100276
dc.rights.licenseCC BY
melbourne.elementsid1287763
melbourne.contributor.authorHeazlewood, Joshua
melbourne.contributor.authorBacic, Anthony
melbourne.contributor.authorEbert, Berit
melbourne.contributor.authorRautengarten, Carsten
melbourne.contributor.authorZeng, Wei
melbourne.contributor.authorBeahan, Cherie
dc.identifier.eissn1460-2431
melbourne.identifier.fundernameidAustralian Research Council, FT130101165
melbourne.identifier.fundernameidAustralian Research Council, FT160100276
melbourne.accessrightsOpen Access


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