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    Cytokine profiling in healthy children shows association of age with cytokine concentrations.

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    Author
    Decker, M-L; Gotta, V; Wellmann, S; Ritz, N
    Date
    2017-12-19
    Source Title
    Scientific Reports
    Publisher
    Springer Science and Business Media LLC
    University of Melbourne Author/s
    Ritz, Nicole
    Affiliation
    Paediatrics (RCH)
    Metadata
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    Document Type
    Journal Article
    Citations
    Decker, M. -L., Gotta, V., Wellmann, S. & Ritz, N. (2017). Cytokine profiling in healthy children shows association of age with cytokine concentrations.. Sci Rep, 7 (1), pp.17842-. https://doi.org/10.1038/s41598-017-17865-2.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/255582
    DOI
    10.1038/s41598-017-17865-2
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736560
    Abstract
    Cytokine-based diagnostic assays are increasingly used in research and clinical practice. Assays developed for adults such as the interferon-gamma release assay for tuberculosis show inferior performance in children. Limited evidence suggests that release of cytokines is influenced by age but normal ranges of cytokines in children are lacking. Whole blood of healthy children (0-12 years) undergoing elective/diagnostic procedures was stimulated with SEB, PHA, Candida albicans for 24 hours or left unstimulated. Concentrations of eight cytokines were measured by multiplex bead-based immunoassays and associations with age and other factors quantified by regression analysis. 271 children (median age 5.2 years) were included. In unstimulated samples IL-1ra, IP-10 and TNF-α concentrations decreased by up to -60% with age. Following antigen stimulation, an age-associated increase (ranging from +90% to +500%) was observed for all cytokines except IL-1ra (significant for IL-4, IFN-γ and TNF-α). Inter-individual variability in cytokine concentrations was large with a coefficient of variation ranging from 42% to 1412%. Despite inter-individual variation age was identified as a strong influencing factor of cytokine concentrations. Age-specific normal values need to be considered for cytokine-based diagnostic purposes. These results are relevant for development of novel cytokine-based diagnostic assays and for optimal dosing of therapeutic agents targeting cytokines.

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