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dc.contributor.authorCyrklaff, M
dc.contributor.authorKudryashev, M
dc.contributor.authorLeis, A
dc.contributor.authorLeonard, K
dc.contributor.authorBaumeister, W
dc.contributor.authorMenard, R
dc.contributor.authorMeissner, M
dc.contributor.authorFrischknecht, F
dc.date.accessioned2020-12-18T03:01:24Z
dc.date.available2020-12-18T03:01:24Z
dc.date.issued2007-06-11
dc.identifierpii: jem.20062405
dc.identifier.citationCyrklaff, M., Kudryashev, M., Leis, A., Leonard, K., Baumeister, W., Menard, R., Meissner, M. & Frischknecht, F. (2007). Cryoelectron tomography reveals periodic material at the inner side of subpellicular microtubules in apicomplexan parasites. JOURNAL OF EXPERIMENTAL MEDICINE, 204 (6), pp.1281-1287. https://doi.org/10.1084/jem.20062405.
dc.identifier.issn0022-1007
dc.identifier.urihttp://hdl.handle.net/11343/255596
dc.description.abstractMicrotubules are dynamic cytoskeletal structures important for cell division, polarity, and motility and are therefore major targets for anticancer and antiparasite drugs. In the invasive forms of apicomplexan parasites, which are highly polarized and often motile cells, exceptionally stable subpellicular microtubules determine the shape of the parasite, and serve as tracks for vesicle transport. We used cryoelectron tomography to image cytoplasmic structures in three dimensions within intact, rapidly frozen Plasmodium sporozoites. This approach revealed microtubule walls that are extended at the luminal side by an additional 3 nm compared to microtubules of mammalian cells. Fourier analysis revealed an 8-nm longitudinal periodicity of the luminal constituent, suggesting the presence of a molecule interacting with tubulin dimers. In silico generation and analysis of microtubule models confirmed this unexpected topology. Microtubules from extracted sporozoites and Toxoplasma gondii tachyzoites showed a similar density distribution, suggesting that the putative protein is conserved among Apicomplexa and serves to stabilize microtubules.
dc.languageEnglish
dc.publisherROCKEFELLER UNIV PRESS
dc.titleCryoelectron tomography reveals periodic material at the inner side of subpellicular microtubules in apicomplexan parasites
dc.typeJournal Article
dc.identifier.doi10.1084/jem.20062405
melbourne.affiliation.departmentBio21
melbourne.source.titleJournal of Experimental Medicine
melbourne.source.volume204
melbourne.source.issue6
melbourne.source.pages1281-1287
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1287813
melbourne.contributor.authorLeis, Andrew
dc.identifier.eissn1540-9538
melbourne.accessrightsOpen Access


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