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    An orthotopic mouse model of gastric cancer invasion and metastasis

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    Author
    Busuttil, RA; Liu, DS; Di Costanzo, N; Schroder, J; Mitchell, C; Boussioutas, A
    Date
    2018-01-16
    Source Title
    Scientific Reports
    Publisher
    NATURE PUBLISHING GROUP
    University of Melbourne Author/s
    Schroeder, Jan; Busuttil, Rita; Boussioutas, Alex
    Affiliation
    Medicine and Radiology
    Computing and Information Systems
    Metadata
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    Document Type
    Journal Article
    Citations
    Busuttil, R. A., Liu, D. S., Di Costanzo, N., Schroder, J., Mitchell, C. & Boussioutas, A. (2018). An orthotopic mouse model of gastric cancer invasion and metastasis. SCIENTIFIC REPORTS, 8 (1), https://doi.org/10.1038/s41598-017-19025-y.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/255619
    DOI
    10.1038/s41598-017-19025-y
    Abstract
    Gastric cancer is a leading cause of cancer death worldwide, with advanced stage being correlated to the level of tumour invasion and metastasis. Current research is heavily focused on the identification and development of efficacious therapeutics targeting these fundamental hallmarks of cancer, however there are currently no animal models that mimic the invasive phenotypes observed in humans. To address this we have developed an orthotopic mouse model whereby gastric cancer cell lines are tagged with luciferase and injected into the subserosal layer of the stomach. This allows for the monitoring of primary tumour growth and metastasis in real-time as well as quantitation of the degree of tumour invasion through the stomach wall by immunohistochemistry. We have three models based on the degree of invasion and metastasis that are cell line specific: The AGS cells develop into invasive tumours by 4-weeks with no evidence of metastases, MKN45 cells are moderately metastatic with minimal invasion till week 2 and MKN28 cells are highly metastatic and fully invasive by week 1. These models have utility as a tool for testing the efficacy of anti-tumour, anti-invasive and anti-metastatic therapies in the setting of gastric cancer, which currently has poor treatment options.

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