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dc.contributor.authorCounihan, NA
dc.contributor.authorChisholm, SA
dc.contributor.authorBullen, HE
dc.contributor.authorSrivastava, A
dc.contributor.authorSanders, PR
dc.contributor.authorJonsdottir, TK
dc.contributor.authorWeiss, GE
dc.contributor.authorGhosh, S
dc.contributor.authorCrabb, BS
dc.contributor.authorCreek, DJ
dc.contributor.authorGilson, PR
dc.contributor.authorde Koning-Ward, TF
dc.date.accessioned2020-12-18T03:14:04Z
dc.date.available2020-12-18T03:14:04Z
dc.date.issued2017-03-02
dc.identifier.citationCounihan, N. A., Chisholm, S. A., Bullen, H. E., Srivastava, A., Sanders, P. R., Jonsdottir, T. K., Weiss, G. E., Ghosh, S., Crabb, B. S., Creek, D. J., Gilson, P. R. & de Koning-Ward, T. F. (2017). Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate. ELIFE, 6, https://doi.org/10.7554/eLife.23217.
dc.identifier.issn2050-084X
dc.identifier.urihttp://hdl.handle.net/11343/255686
dc.description.abstractPlasmodium falciparum parasites, the causative agents of malaria, modify their host erythrocyte to render them permeable to supplementary nutrient uptake from the plasma and for removal of toxic waste. Here we investigate the contribution of the rhoptry protein RhopH2, in the formation of new permeability pathways (NPPs) in Plasmodium-infected erythrocytes. We show RhopH2 interacts with RhopH1, RhopH3, the erythrocyte cytoskeleton and exported proteins involved in host cell remodeling. Knockdown of RhopH2 expression in cycle one leads to a depletion of essential vitamins and cofactors and decreased de novo synthesis of pyrimidines in cycle two. There is also a significant impact on parasite growth, replication and transition into cycle three. The uptake of solutes that use NPPs to enter erythrocytes is also reduced upon RhopH2 knockdown. These findings provide direct genetic support for the contribution of the RhopH complex in NPP activity and highlight the importance of NPPs to parasite survival.
dc.languageEnglish
dc.publisherELIFE SCIENCES PUBLICATIONS LTD
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titlePlasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate
dc.typeJournal Article
dc.identifier.doi10.7554/eLife.23217
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titleeLife
melbourne.source.volume6
dc.rights.licenseCC BY
melbourne.elementsid1196892
melbourne.contributor.authorCrabb, Brendan
melbourne.contributor.authorChisholm, Scott
dc.identifier.eissn2050-084X
melbourne.accessrightsOpen Access


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