A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling.
University of Melbourne Author/sVerberne, Antonius
AffiliationMedicine (Austin & Northern Health)
Document TypeJournal Article
CitationsVerberne, A. J. M. (2016). A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling.. Bioengineering (Basel), 3 (4), pp.27-27. https://doi.org/10.3390/bioengineering3040027.
Access StatusOpen Access
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597270
Juxtacellular neuronal labelling is a method which allows neurophysiologists to fill physiologically-identified neurons with small positively-charged marker molecules. Labelled neurons are identified by histochemical processing of brain sections along with immunohistochemical identification of neuropeptides, neurotransmitters, neurotransmitter transporters or biosynthetic enzymes. A microcontroller-based pulser circuit and associated BASIC software script is described for incorporation into the design of a commercially-available intracellular electrometer for use in juxtacellular neuronal labelling. Printed circuit board construction has been used for reliability and reproducibility. The current design obviates the need for a separate digital pulse source and simplifies the juxtacellular neuronal labelling procedure.
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