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    A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling.

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    Author
    Verberne, AJM
    Date
    2016-10-19
    Source Title
    Bioengineering
    Publisher
    MDPI AG
    University of Melbourne Author/s
    Verberne, Antonius
    Affiliation
    Medicine and Radiology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Verberne, A. J. M. (2016). A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling.. Bioengineering (Basel), 3 (4), pp.27-27. https://doi.org/10.3390/bioengineering3040027.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/255699
    DOI
    10.3390/bioengineering3040027
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597270
    Abstract
    Juxtacellular neuronal labelling is a method which allows neurophysiologists to fill physiologically-identified neurons with small positively-charged marker molecules. Labelled neurons are identified by histochemical processing of brain sections along with immunohistochemical identification of neuropeptides, neurotransmitters, neurotransmitter transporters or biosynthetic enzymes. A microcontroller-based pulser circuit and associated BASIC software script is described for incorporation into the design of a commercially-available intracellular electrometer for use in juxtacellular neuronal labelling. Printed circuit board construction has been used for reliability and reproducibility. The current design obviates the need for a separate digital pulse source and simplifies the juxtacellular neuronal labelling procedure.

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