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dc.contributor.authorFink, PJ
dc.contributor.authorSwan, K
dc.contributor.authorTurk, G
dc.contributor.authorMoore, MW
dc.contributor.authorCarbone, FR
dc.date.accessioned2020-12-18T03:19:47Z
dc.date.available2020-12-18T03:19:47Z
dc.date.issued1992-12-01
dc.identifier.citationFink, P. J., Swan, K., Turk, G., Moore, M. W. & Carbone, F. R. (1992). Both intrathymic and peripheral selection modulate the differential expression of V beta 5 among CD4+ and CD8+ T cells.. J Exp Med, 176 (6), pp.1733-1738. https://doi.org/10.1084/jem.176.6.1733.
dc.identifier.issn0022-1007
dc.identifier.urihttp://hdl.handle.net/11343/255723
dc.description.abstractMurine T cells expressing V beta 5 are characterized by (a) intrathymic deletion in the presence of I-E and products of endogenous mouse mammary tumor viruses, and (b) a greater representation in CD8+ relative to CD4+ peripheral T cells, thought to be due to more efficient intrathymic positive selection on class I rather than class II major histocompatibility complex antigens. We have engineered mice that are transgenic for a rearranged gene encoding a V beta 5+ beta chain of the T cell receptor for antigen. Deletion is not predicted in I-E- V beta 5+ transgenic mice, and until the age of 2 wk, the CD4/CD8 ratio of peripheral T cells is > 3:1 and indistinguishable between transgenic and nontransgenic mice. Transgenic mice then show a rapid, age-dependent decline in the ratio of CD4+ to CD8+ T cells in the lymphoid periphery, reaching a low of 1:10 by 7 mo of age. Furthermore, the percent of peripheral CD4+ cells that express the transgene drops with age, reaching a low of about 60% at 7 mo, while the percent of CD8+ cells that express V beta 5 remains greater than 95% at all ages. The lymphoid periphery is implicated in this selection against CD4+ V beta 5+ T cells as it occurs more rapidly in thymectomized transgenic mice, and can be delayed in mice whose peripheral T cells are replaced by recent thymic emigrants after depletion by in vivo treatment with anti-Thy-1 antibodies. These results indicate that the relative expression of V beta 5 in T cell subsets can be influenced not only intrathymically in I-E+ V beta 5+ transgenic mice, but also by events in the periphery, in the absence of I-E expression.
dc.languageeng
dc.publisherRockefeller University Press
dc.titleBoth intrathymic and peripheral selection modulate the differential expression of V beta 5 among CD4+ and CD8+ T cells.
dc.typeJournal Article
dc.identifier.doi10.1084/jem.176.6.1733
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titleJournal of Experimental Medicine
melbourne.source.volume176
melbourne.source.issue6
melbourne.source.pages1733-1738
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1296802
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119442
melbourne.contributor.authorCarbone, Francis
dc.identifier.eissn1540-9538
melbourne.accessrightsOpen Access


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