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dc.contributor.authorBoyle, AG
dc.contributor.authorRankin, SC
dc.contributor.authorDuffee, L
dc.contributor.authorBoston, RC
dc.contributor.authorWheeler-Aceto, H
dc.date.accessioned2020-12-18T03:40:29Z
dc.date.available2020-12-18T03:40:29Z
dc.date.issued2016-01
dc.identifier.citationBoyle, A. G., Rankin, S. C., Duffee, L., Boston, R. C. & Wheeler-Aceto, H. (2016). Streptococcus equi Detection Polymerase Chain Reaction Assay for Equine Nasopharyngeal and Guttural Pouch Wash Samples.. J Vet Intern Med, 30 (1), pp.276-281. https://doi.org/10.1111/jvim.13808.
dc.identifier.issn0891-6640
dc.identifier.urihttp://hdl.handle.net/11343/255871
dc.description.abstractBACKGROUND: Bacterial culture and polymerase chain reaction (PCR) assays for the detection of Streptococcus equi in nasopharyngeal washes (NPW) and guttural pouch lavage (GPL) samples have low sensitivity. In human diagnostics, processing of samples with flocked swabs has improved recovery rates of bacterial agents because of improved surface area and elution factors. HYPOTHESIS: For S. equi subsp. equi (S. equi) detection in NPW and GPL samples we hypothesized that: direct-PCR would be more reliable than flocked swab culture (FS culture); flocked swab PCR (FS-PCR) would be equivalent to direct-PCR; and FS culture would be more reliable than traditional culture. SAMPLES: A total of 193 samples (134 NPW and 59 GPL) from 113 horses with either suspected S. equi infection, convalescing from a known S. equi infection, or asymptomatic horses screened for S. equi. METHODS: Prospective study. Samples were submitted for S. equi direct-PCR. Using logistic regression, direct-PCR (gold standard) was compared to FS culture, traditional culture, and FS-PCR also performed. RESULTS: Direct-PCR was statistically more sensitive than FS-PCR, FS culture, and traditional culture (P < .001). All methods had sensitivities <70% relative to the direct-PCR. FS culture had a similar sensitivity relative to traditional culture. The odds of GPL samples being positive on direct-PCR (P = .030) and FS-PCR were greater than those for NPW samples (P = .021). CONCLUSIONS AND CLINICAL IMPORTANCE: Use of flocked swabs during laboratory preprocessing did not improve detection of S. equi via either PCR or bacterial culture from samples. Direct-PCR is the preferred method of detection of S. equi.
dc.languageeng
dc.publisherWiley
dc.titleStreptococcus equi Detection Polymerase Chain Reaction Assay for Equine Nasopharyngeal and Guttural Pouch Wash Samples.
dc.typeJournal Article
dc.identifier.doi10.1111/jvim.13808
melbourne.affiliation.departmentMedicine and Radiology
melbourne.source.titleJournal of Veterinary Internal Medicine
melbourne.source.volume30
melbourne.source.issue1
melbourne.source.pages276-281
dc.rights.licenseCC BY-NC
melbourne.elementsid1302082
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913660
melbourne.contributor.authorBoston, Raymond
dc.identifier.eissn1939-1676
melbourne.accessrightsOpen Access


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