Single cell RNA sequencing of stem cell-derived retinal ganglion cells

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    Author
    Daniszewski, MB; Senabouth, A; Nguyen, QH; Crombie, DE; Lukowski, SW; Kulkarni, T; Sluch, VM; Jabbari, JS; Chamling, X; Zack, DJ; ...
    Date
    2018-02-13
    Source Title
    Scientific Data
    Publisher
    NATURE PUBLISHING GROUP
    University of Melbourne Author/s
    Pebay, Alice; Crombie, Duncan; Daniszewski, Maciej
    Affiliation
    Surgery (RMH)
    Florey Department of Neuroscience and Mental Health
    Anatomy and Neuroscience
    Metadata
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    Document Type
    Journal Article
    Citations
    Daniszewski, M. B., Senabouth, A., Nguyen, Q. H., Crombie, D. E., Lukowski, S. W., Kulkarni, T., Sluch, V. M., Jabbari, J. S., Chamling, X., Zack, D. J., Pebay, A., Powell, J. E. & Hewitt, A. W. (2018). Single cell RNA sequencing of stem cell-derived retinal ganglion cells. SCIENTIFIC DATA, 5 (1), https://doi.org/10.1038/sdata.2018.13.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/255911
    DOI
    10.1038/sdata.2018.13
    ARC Grant code
    ARC/FT140100047
    Abstract
    We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells showed increased expression of genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature RGC phenotype.

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