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dc.contributor.authorPenington, JS
dc.contributor.authorPenno, MAS
dc.contributor.authorNgui, KM
dc.contributor.authorAjami, NJ
dc.contributor.authorRoth-Schulze, AJ
dc.contributor.authorWilcox, SA
dc.contributor.authorBandala-Sanchez, E
dc.contributor.authorWentworth, JM
dc.contributor.authorBarry, SC
dc.contributor.authorBrown, CY
dc.contributor.authorCouper, JJ
dc.contributor.authorPetrosino, JF
dc.contributor.authorPapenfuss, AT
dc.contributor.authorHarrison, LC
dc.date.accessioned2020-12-18T04:16:39Z
dc.date.available2020-12-18T04:16:39Z
dc.date.issued2018-03-12
dc.identifierpii: 10.1038/s41598-018-22491-7
dc.identifier.citationPenington, J. S., Penno, M. A. S., Ngui, K. M., Ajami, N. J., Roth-Schulze, A. J., Wilcox, S. A., Bandala-Sanchez, E., Wentworth, J. M., Barry, S. C., Brown, C. Y., Couper, J. J., Petrosino, J. F., Papenfuss, A. T. & Harrison, L. C. (2018). Influence of fecal collection conditions and 16S rRNA gene sequencing at two centers on human gut microbiota analysis. SCIENTIFIC REPORTS, 8 (1), https://doi.org/10.1038/s41598-018-22491-7.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/256125
dc.description.abstractTo optimise fecal sampling for reproducible analysis of the gut microbiome, we compared different methods of sample collection and sequencing of 16S rRNA genes at two centers. Samples collected from six individuals on three consecutive days were placed in commercial collection tubes (OMNIgeneGut OMR-200) or in sterile screw-top tubes in a home fridge or home freezer for 6-24 h, before transfer and storage at -80 °C. Replicate samples were shipped to centers in Australia and the USA for DNA extraction and sequencing by their respective PCR protocols, and analysed with the same bioinformatic pipeline. Variation in gut microbiome was dominated by differences between individuals. Minor differences in the abundance of taxa were found between collection-processing methods and day of collection, and between the two centers. We conclude that collection with storage and transport at 4 °C within 24 h is adequate for 16S rRNA analysis of the gut microbiome. Other factors including differences in PCR and sequencing methods account for relatively minor variation compared to differences between individuals.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.titleInfluence of fecal collection conditions and 16S rRNA gene sequencing at two centers on human gut microbiota analysis
dc.typeJournal Article
dc.identifier.doi10.1038/s41598-018-22491-7
melbourne.affiliation.departmentMedicine and Radiology
melbourne.affiliation.departmentMedical Education
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.affiliation.departmentComputing and Information Systems
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.source.titleScientific Reports
melbourne.source.volume8
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1313936
melbourne.contributor.authorPapenfuss, Anthony
melbourne.contributor.authorHarrison, Leonard
melbourne.contributor.authorBandala Sanchez, Maria
melbourne.contributor.authorWilcox, Stephen
melbourne.contributor.authorWentworth, John
melbourne.contributor.authorSinnott, Richard
melbourne.contributor.authorVuillermin, Peter
melbourne.contributor.authorRoth Schulze, Alexandra
melbourne.contributor.authorColman, Peter
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


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