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dc.contributor.authorDefraine, V
dc.contributor.authorLiebens, V
dc.contributor.authorLoos, E
dc.contributor.authorSwings, T
dc.contributor.authorWeytjens, B
dc.contributor.authorFierro, C
dc.contributor.authorMarchal, K
dc.contributor.authorSharkey, L
dc.contributor.authorO'Neill, AJ
dc.contributor.authorCorbau, R
dc.contributor.authorMarchand, A
dc.contributor.authorChaltin, P
dc.contributor.authorFauvart, M
dc.contributor.authorMichiels, J
dc.date.accessioned2020-12-18T04:19:21Z
dc.date.available2020-12-18T04:19:21Z
dc.date.issued2018-02-08
dc.identifier.citationDefraine, V., Liebens, V., Loos, E., Swings, T., Weytjens, B., Fierro, C., Marchal, K., Sharkey, L., O'Neill, A. J., Corbau, R., Marchand, A., Chaltin, P., Fauvart, M. & Michiels, J. (2018). 1-((2,4-Dichlorophenethyl)Amino)3-Phenoxypropan-2-ol Kills Pseudomonas aeruginosa through Extensive Membrane Damage. FRONTIERS IN MICROBIOLOGY, 9 (FEB), https://doi.org/10.3389/fmicb.2018.00129.
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/11343/256144
dc.description.abstractThe ever increasing multidrug-resistance of clinically important pathogens and the lack of novel antibiotics have resulted in a true antibiotic crisis where many antibiotics are no longer effective. Further complicating the treatment of bacterial infections are antibiotic-tolerant persister cells. Besides being responsible for the recalcitrant nature of chronic infections, persister cells greatly contribute to the observed antibiotic tolerance in biofilms and even facilitate the emergence of antibiotic resistance. Evidently, eradication of these persister cells could greatly improve patient outcomes and targeting persistence may provide an alternative approach in combatting chronic infections. We recently characterized 1-((2,4-dichlorophenethyl)amino)-3-phenoxypropan-2-ol (SPI009), a novel anti-persister molecule capable of directly killing persisters from both Gram-negative and Gram-positive pathogens. SPI009 potentiates antibiotic activity in several in vitro and in vivo infection models and possesses promising anti-biofilm activity. Strikingly, SPI009 restores antibiotic sensitivity even in resistant strains. In this study, we investigated the mode of action of this novel compound using several parallel approaches. Genetic analyses and a macromolecular synthesis assays suggest that SPI009 acts by causing extensive membrane damage. This hypothesis was confirmed by liposome leakage assay and membrane permeability studies, demonstrating that SPI009 rapidly impairs the bacterial outer and inner membranes. Evaluation of SPI009-resistant mutants, which only could be generated under severe selection pressure, suggested a possible role for the MexCD-OprJ efflux pump. Overall, our results demonstrate the extensive membrane-damaging activity of SPI009 and confirm its clinical potential in the development of novel anti-persister therapies.
dc.languageEnglish
dc.publisherFRONTIERS MEDIA SA
dc.title1-((2,4-Dichlorophenethyl)Amino)3-Phenoxypropan-2-ol Kills Pseudomonas aeruginosa through Extensive Membrane Damage
dc.typeJournal Article
dc.identifier.doi10.3389/fmicb.2018.00129
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titleFrontiers in Microbiology
melbourne.source.volume9
melbourne.source.issueFEB
dc.rights.licenseCC BY
melbourne.elementsid1314143
melbourne.contributor.authorSharkey, Liam
dc.identifier.eissn1664-302X
melbourne.accessrightsOpen Access


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