Show simple item record

dc.contributor.authorAlsaweed, M
dc.contributor.authorHepworth, AR
dc.contributor.authorLefevre, C
dc.contributor.authorHartmann, PE
dc.contributor.authorGeddes, DT
dc.contributor.authorHassiotou, F
dc.date.accessioned2020-12-18T04:20:24Z
dc.date.available2020-12-18T04:20:24Z
dc.date.issued2015-10-01
dc.identifier.citationAlsaweed, M., Hepworth, A. R., Lefevre, C., Hartmann, P. E., Geddes, D. T. & Hassiotou, F. (2015). Human Milk MicroRNA and Total RNA Differ Depending on Milk Fractionation. JOURNAL OF CELLULAR BIOCHEMISTRY, 116 (10), pp.2397-2407. https://doi.org/10.1002/jcb.25207.
dc.identifier.issn0730-2312
dc.identifier.urihttp://hdl.handle.net/11343/256151
dc.description.abstractMicroRNA have been recently discovered in human milk signifying potentially important functions for both the lactating breast and the infant. Whilst human milk microRNA have started to be explored, little data exist on the evaluation of sample processing, and analysis to ensure that a full spectrum of microRNA can be obtained. Human milk comprises three main fractions: cells, skim milk, and lipids. Typically, the skim milk fraction has been measured in isolation despite evidence that the lipid fraction may contain more microRNA. This study aimed to standardize isolation of microRNA and total RNA from all three fractions of human milk to determine the most appropriate sampling and analysis procedure for future studies. Three different methods from eight commercially available kits were tested for their efficacy in extracting total RNA and microRNA from the lipid, skim, and cell fractions of human milk. Each fraction yielded different concentrations of RNA and microRNA, with the highest quantities found in the cell and lipid fractions, and the lowest in skim milk. The column-based phenol-free method was the most efficient extraction method for all three milk fractions. Two microRNAs were expressed and validated in the three milk fractions by qPCR using the three recommended extraction kits for each fraction. High expression levels were identified in the skim and lipid milk factions for these microRNAs. These results suggest that careful consideration of both the human milk sample preparation and extraction protocols should be made prior to embarking upon research in this area.
dc.languageEnglish
dc.publisherWILEY-BLACKWELL
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.titleHuman Milk MicroRNA and Total RNA Differ Depending on Milk Fractionation
dc.typeJournal Article
dc.identifier.doi10.1002/jcb.25207
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleJournal of Cellular Biochemistry
melbourne.source.volume116
melbourne.source.issue10
melbourne.source.pages2397-2407
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1315753
melbourne.contributor.authorLefevre, Christophe
dc.identifier.eissn1097-4644
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record