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dc.contributor.authorParsons, LM
dc.contributor.authorGrzeschik, NA
dc.contributor.authorAmaratunga, K
dc.contributor.authorBurke, P
dc.contributor.authorQuinn, LM
dc.contributor.authorRichardson, HE
dc.date.accessioned2020-12-18T04:27:34Z
dc.date.available2020-12-18T04:27:34Z
dc.date.issued2017-08-01
dc.identifierpii: g3.117.043513
dc.identifier.citationParsons, L. M., Grzeschik, N. A., Amaratunga, K., Burke, P., Quinn, L. M. & Richardson, H. E. (2017). A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues. G3-GENES GENOMES GENETICS, 7 (8), pp.2497-2509. https://doi.org/10.1534/g3.117.043513.
dc.identifier.issn2160-1836
dc.identifier.urihttp://hdl.handle.net/11343/256202
dc.description.abstractIn both Drosophila melanogaster and mammalian systems, epithelial structure and underlying cell polarity are essential for proper tissue morphogenesis and organ growth. Cell polarity interfaces with multiple cellular processes that are regulated by the phosphorylation status of large protein networks. To gain insight into the molecular mechanisms that coordinate cell polarity with tissue growth, we screened a boutique collection of RNAi stocks targeting the kinome for their capacity to modify Drosophila "cell polarity" eye and wing phenotypes. Initially, we identified kinase or phosphatase genes whose depletion modified adult eye phenotypes associated with the manipulation of cell polarity complexes (via overexpression of Crb or aPKC). We next conducted a secondary screen to test whether these cell polarity modifiers altered tissue overgrowth associated with depletion of Lgl in the wing. These screens identified Hippo, Jun kinase (JNK), and Notch signaling pathways, previously linked to cell polarity regulation of tissue growth. Furthermore, novel pathways not previously connected to cell polarity regulation of tissue growth were identified, including Wingless (Wg/Wnt), Ras, and lipid/Phospho-inositol-3-kinase (PI3K) signaling pathways. Additionally, we demonstrated that the "nutrient sensing" kinases Salt Inducible Kinase 2 and 3 (SIK2 and 3) are potent modifiers of cell polarity phenotypes and regulators of tissue growth. Overall, our screen has revealed novel cell polarity-interacting kinases and phosphatases that affect tissue growth, providing a platform for investigating molecular mechanisms coordinating cell polarity and tissue growth during development.
dc.languageEnglish
dc.publisherGENETICS SOCIETY AMERICA
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleA Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues
dc.typeJournal Article
dc.identifier.doi10.1534/g3.117.043513
melbourne.affiliation.departmentAnatomy and Neuroscience
melbourne.source.titleG3: Genes, Genomes, Genetics
melbourne.source.volume7
melbourne.source.issue8
melbourne.source.pages2497-2509
melbourne.identifier.nhmrc628401
melbourne.identifier.nhmrc1020056
dc.rights.licenseCC BY
melbourne.elementsid1215033
melbourne.contributor.authorRichardson, Helena
melbourne.contributor.authorParsons, Linda
melbourne.contributor.authorQuinn, Leonie
melbourne.contributor.authorBurke, Peter
dc.identifier.eissn2160-1836
melbourne.identifier.fundernameidNHMRC, 628401
melbourne.identifier.fundernameidNHMRC, 1020056
melbourne.accessrightsOpen Access


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