WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells

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    Author
    Alshawaf, AJ; Antonic, A; Skafidas, E; Ng, DC-H; Dottori, M
    Date
    2017-01-01
    Source Title
    Stem Cells International
    Publisher
    HINDAWI LTD
    University of Melbourne Author/s
    Dottori, Mirella; Skafidas, Efstratios; Antonic-Baker, Ana; Alshawaf, Abdullah
    Affiliation
    Electrical and Electronic Engineering
    Biomedical Engineering
    Florey Department of Neuroscience and Mental Health
    Metadata
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    Document Type
    Journal Article
    Citations
    Alshawaf, A. J., Antonic, A., Skafidas, E., Ng, D. C. -H. & Dottori, M. (2017). WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells. STEM CELLS INTERNATIONAL, 2017, https://doi.org/10.1155/2017/7848932.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/256314
    DOI
    10.1155/2017/7848932
    Abstract
    Mutations in WD40-repeat protein 62 (WDR62) are commonly associated with primary microcephaly and other developmental cortical malformations. We used human pluripotent stem cells (hPSC) to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis. Neurospheres lacking WDR62 expression showed decreased expression of intermediate progenitor marker, TBR2, and also glial marker, S100β. In contrast, inhibition of c-Jun N-terminal kinase (JNK) signalling during hPSC neural differentiation induced upregulation of WDR62 with a corresponding increase in neural and glial progenitor markers, PAX6 and EAAT1, respectively. These findings may signify a role of WDR62 in specifying intermediate neural and glial progenitors during human pluripotent stem cell differentiation.

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