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dc.contributor.authorSeidel, A
dc.contributor.authorYe, Y
dc.contributor.authorde Armas, LR
dc.contributor.authorSoto, M
dc.contributor.authorYarosh, W
dc.contributor.authorMarcsisin, RA
dc.contributor.authorTran, D
dc.contributor.authorSelsted, ME
dc.contributor.authorCamerini, D
dc.date.accessioned2020-12-21T01:08:16Z
dc.date.available2020-12-21T01:08:16Z
dc.date.issued2010-03-17
dc.identifier.citationSeidel, A., Ye, Y., de Armas, L. R., Soto, M., Yarosh, W., Marcsisin, R. A., Tran, D., Selsted, M. E. & Camerini, D. (2010). Cyclic and acyclic defensins inhibit human immunodeficiency virus type-1 replication by different mechanisms.. PLoS One, 5 (3), pp.e9737-. https://doi.org/10.1371/journal.pone.0009737.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/256383
dc.description.abstractDefensins are antimicrobial peptides expressed by plants and animals. In mammals there are three subfamilies of defensins, distinguished by structural features: alpha, beta and theta. Alpha and beta-defensins are linear peptides with broad anti-microbial activity that are expressed by many mammals including humans. In contrast, theta-defensins are cyclic anti-microbial peptides made by several non-human primates but not humans. All three defensin types have anti-HIV-1 activity, but their mechanisms of action differ. We studied the anti-HIV-1 activity of one defensin from each group, HNP-1 (alpha), HBD-2 (beta) and RTD-1 (theta). We examined how each defensin affected HIV-1 infection and demonstrated that the cyclic defensin RTD-1 inhibited HIV-1 entry, while acyclic HNP-1 and HBD-2 inhibited HIV-1 replication even when added 12 hours post-infection and blocked viral replication after HIV-1 cDNA formation. We further found that all three defensins downmodulated CXCR4. Moreover, RTD-1 inactivated X4 HIV-1, while HNP-1 and HBD-2 inactivated both X4 and R5 HIV-1. The data presented here show that acyclic and cyclic defensins block HIV-1 replication by shared and diverse mechanisms. Moreover, we found that HNP-1 and RTD-1 directly inhibited firefly luciferase enzymatic activity, which may affect the interpretation of previously published data.
dc.languageeng
dc.publisherPublic Library of Science (PLoS)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleCyclic and acyclic defensins inhibit human immunodeficiency virus type-1 replication by different mechanisms.
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0009737
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titlePLoS One
melbourne.source.volume5
melbourne.source.issue3
melbourne.source.pagese9737-
dc.rights.licenseCC BY
melbourne.elementsid1220489
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840026
melbourne.contributor.authorMARCSISIN, RENEE
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


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