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dc.contributor.authorLoi, M
dc.contributor.authorCenni, V
dc.contributor.authorDuchi, S
dc.contributor.authorSquarzoni, S
dc.contributor.authorLopez-Otin, C
dc.contributor.authorFoisner, R
dc.contributor.authorLattanzi, G
dc.contributor.authorCapanni, C
dc.date.accessioned2020-12-21T01:29:45Z
dc.date.available2020-12-21T01:29:45Z
dc.date.issued2016-03-29
dc.identifierpii: 6697
dc.identifier.citationLoi, M., Cenni, V., Duchi, S., Squarzoni, S., Lopez-Otin, C., Foisner, R., Lattanzi, G. & Capanni, C. (2016). Barrier-to-Autointegration Factor (BAF) involvement in prelamin A-related chromatin organization changes. ONCOTARGET, 7 (13), pp.15662-15677. https://doi.org/10.18632/oncotarget.6697.
dc.identifier.issn1949-2553
dc.identifier.urihttp://hdl.handle.net/11343/256535
dc.description.abstractChromatin disorganization is one of the major alterations linked to prelamin A processing impairment. In this study we demonstrate that BAF is necessary to modulate prelamin A effects on chromatin structure. We show that when prelamin A and BAF cannot properly interact no prelamin A-dependent effects on chromatin occur; similar to what is observed in human Nestor Guillermo Progeria Syndrome cells harboring a BAF mutation, in HEK293 cells expressing a BAF mutant unable to bind prelamin A, or in siRNA mediated BAF-depleted HEK293 cells expressing prelamin A. BAF is necessary to induce histone trimethyl-H3K9 as well as HP1-alpha and LAP2-alpha nuclear relocalization in response to prelamin A accumulation. These findings are enforced by electron microscopy evaluations showing how the prelamin A-BAF interaction governs overall chromatin organization. Finally, we demonstrate that the LAP2-alpha nuclear localization defect observed in HGPS cells involves the progerin-BAF interaction, thus establishing a functional link between BAF and prelamin A pathological forms.
dc.languageEnglish
dc.publisherIMPACT JOURNALS LLC
dc.titleBarrier-to-Autointegration Factor (BAF) involvement in prelamin A-related chromatin organization changes
dc.typeJournal Article
dc.identifier.doi10.18632/oncotarget.6697
melbourne.affiliation.departmentSurgery (St Vincent's)
melbourne.source.titleOncotarget
melbourne.source.volume7
melbourne.source.issue13
melbourne.source.pages15662-15677
dc.rights.licenseCC BY
melbourne.elementsid1223378
melbourne.contributor.authorDuchi, Serena
dc.identifier.eissn1949-2553
melbourne.accessrightsOpen Access


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