Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells

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    Author
    Bulj, Z; Duchi, S; Bevilacqua, A; Gherardi, A; Dozza, B; Piccinini, F; Mariani, GA; Lucarelli, E; Giannini, S; Donati, D; ...
    Date
    2013-01-01
    Source Title
    International Journal of Oncology
    Publisher
    SPANDIDOS PUBL LTD
    University of Melbourne Author/s
    Duchi, Serena
    Affiliation
    Surgery (St Vincent's)
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    Document Type
    Journal Article
    Citations
    Bulj, Z., Duchi, S., Bevilacqua, A., Gherardi, A., Dozza, B., Piccinini, F., Mariani, G. A., Lucarelli, E., Giannini, S., Donati, D. & Marmiroli, S. (2013). Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells. INTERNATIONAL JOURNAL OF ONCOLOGY, 42 (1), pp.118-126. https://doi.org/10.3892/ijo.2012.1700.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/256536
    DOI
    10.3892/ijo.2012.1700
    Abstract
    This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for cell migration and invasion. The tests that were implemented revealed that MSC were fairly migratory. Protein kinase B (AKT) was strongly activated at the basal level. Through our analyses we demonstrated that pharmacological inactivation of AKT2 but not AKT1 significantly decreased cell migration and invasion. Although preliminary, collectively our results indicate that AKT2 activation plays a critical role in enabling MSC migration.

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