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dc.contributor.authorLundberg, K
dc.contributor.authorHeath, W
dc.contributor.authorKöntgen, F
dc.contributor.authorCarbone, FR
dc.contributor.authorShortman, K
dc.date.accessioned2020-12-21T01:39:46Z
dc.date.available2020-12-21T01:39:46Z
dc.date.issued1995-05-01
dc.identifier.citationLundberg, K., Heath, W., Köntgen, F., Carbone, F. R. & Shortman, K. (1995). Intermediate steps in positive selection: differentiation of CD4+8int TCRint thymocytes into CD4-8+TCRhi thymocytes.. J Exp Med, 181 (5), pp.1643-1651. https://doi.org/10.1084/jem.181.5.1643.
dc.identifier.issn0022-1007
dc.identifier.urihttp://hdl.handle.net/11343/256605
dc.description.abstractThe differentiation potential of putative intermediates between CD4+8+ thymocytes and mature T cells has been examined. Such intermediate populations were sorted, in parallel with CD4+8+ thymocytes, from three types of C57BL/6 mice: major histocompatibility complex (MHC) class II-deficient mice, mice transgenic for an alpha/beta T cell receptor (TCR) restricted by class I MHC and normal mice. The sorted populations were then transferred into the thymus of nonirradiated C57BL/Ka mice differing in Thy 1 allotype, and the progeny of the transferred cells were analyzed 2 d later. Surprisingly, with all three types of donor mice, a major proportion of the CD4+8intTCRint-derived progeny were found to be CD4-8+TCRhi cells, thus delineating a new alternative pathway for development of the CD8 lineage. In contrast, the transfer of CD4int8+TCRint thymocytes produced CD4-8+TCRhi cells but no significant proportion of CD4+8-TCRhi cells, suggesting that there is no equivalent alternative pathway for the CD4 lineage. The results negate some of the evidence for a stochastic/selective model of lineage commitment, and point to an asymmetry in the steps leading to CD4-8+ versus CD4+8- T cells.
dc.languageeng
dc.publisherRockefeller University Press
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.titleIntermediate steps in positive selection: differentiation of CD4+8int TCRint thymocytes into CD4-8+TCRhi thymocytes.
dc.typeJournal Article
dc.identifier.doi10.1084/jem.181.5.1643
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titleJournal of Experimental Medicine
melbourne.source.volume181
melbourne.source.issue5
melbourne.source.pages1643-1651
dc.rights.licenseCC BY-NC-SA
melbourne.elementsid1226269
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191983
melbourne.contributor.authorHeath, William
melbourne.contributor.authorCarbone, Francis
dc.identifier.eissn1540-9538
melbourne.accessrightsOpen Access


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