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    A rigorous method to enrich for exosomes from brain tissue

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    Author
    Vella, LJ; Scicluna, BJ; Cheng, L; Bawden, EG; Masters, CL; Ang, C-S; Willamson, N; McLean, C; Barnham, KJ; Hill, AF
    Date
    2017-07-26
    Source Title
    Journal of Extracellular Vesicles
    Publisher
    TAYLOR & FRANCIS LTD
    University of Melbourne Author/s
    Ang, Ching-Seng; Vella, Laura; Masters, Colin; Barnham, Kevin; Hill, Andrew; SCICLUNA, BENJAMIN; McLean, Catriona; Williamson, Nicholas
    Affiliation
    Florey Department of Neuroscience and Mental Health
    Bio21
    Biochemistry and Molecular Biology
    Metadata
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    Document Type
    Journal Article
    Citations
    Vella, L. J., Scicluna, B. J., Cheng, L., Bawden, E. G., Masters, C. L., Ang, C. -S., Willamson, N., McLean, C., Barnham, K. J. & Hill, A. F. (2017). A rigorous method to enrich for exosomes from brain tissue. JOURNAL OF EXTRACELLULAR VESICLES, 6 (1), https://doi.org/10.1080/20013078.2017.1348885.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/256624
    DOI
    10.1080/20013078.2017.1348885
    Abstract
    Extracellular vesicles, including exosomes, are released by all cells, including those of the nervous system. Capable of delivering lipid, protein and nucleic acids to both nearby and distal cells, exosomes have been hypothesized to play a role in progression of many diseases of the nervous system. To date, most analyses on the role of these vesicles in the healthy and diseased state have relied on studying vesicles from in vitro sources, such as conditioned cell culture media, or body fluids. Here we have taken a critical approach to the enrichment and characterization of exosomes from human frontal cortex. This method maintains the integrity of the vesicles and their cargo, and comprehensive proteomic and genomic characterization confirms the legitimacy of the resulting extracellular vesicles as endosome-derived exosomes. This method will enable neuroscientists to acquire more detailed information about exosomes in the brain and explore the role(s) this form of intercellular communication and unique source of lipid, protein and RNA has in healthy brain function and pathogenic conditions. Furthermore, this method may have important utility in the isolation of exosomes from other tissues.

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