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    Tentacle Transcriptome and Venom Proteome of the Pacific Sea Nettle, Chrysaora fuscescens (Cnidaria: Scyphozoa)

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    Author
    Ponce, D; Brinkman, DL; Potriquet, J; Mulvenna, J
    Date
    2016-04-01
    Source Title
    Toxins
    Publisher
    MDPI
    University of Melbourne Author/s
    Ponce Garcia, Dalia
    Affiliation
    Pharmacology and Therapeutics
    Metadata
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    Document Type
    Journal Article
    Citations
    Ponce, D., Brinkman, D. L., Potriquet, J. & Mulvenna, J. (2016). Tentacle Transcriptome and Venom Proteome of the Pacific Sea Nettle, Chrysaora fuscescens (Cnidaria: Scyphozoa). TOXINS, 8 (4), https://doi.org/10.3390/toxins8040102.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/256630
    DOI
    10.3390/toxins8040102
    Abstract
    Jellyfish venoms are rich sources of toxins designed to capture prey or deter predators, but they can also elicit harmful effects in humans. In this study, an integrated transcriptomic and proteomic approach was used to identify putative toxins and their potential role in the venom of the scyphozoan jellyfish Chrysaora fuscescens. A de novo tentacle transcriptome, containing more than 23,000 contigs, was constructed and used in proteomic analysis of C. fuscescens venom to identify potential toxins. From a total of 163 proteins identified in the venom proteome, 27 were classified as putative toxins and grouped into six protein families: proteinases, venom allergens, C-type lectins, pore-forming toxins, glycoside hydrolases and enzyme inhibitors. Other putative toxins identified in the transcriptome, but not the proteome, included additional proteinases as well as lipases and deoxyribonucleases. Sequence analysis also revealed the presence of ShKT domains in two putative venom proteins from the proteome and an additional 15 from the transcriptome, suggesting potential ion channel blockade or modulatory activities. Comparison of these potential toxins to those from other cnidarians provided insight into their possible roles in C. fuscescens venom and an overview of the diversity of potential toxin families in cnidarian venoms.

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