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dc.contributor.authorGurung, I
dc.contributor.authorSpielman, I
dc.contributor.authorDavies, MR
dc.contributor.authorLala, R
dc.contributor.authorGaustad, P
dc.contributor.authorBiais, N
dc.contributor.authorPelicic, V
dc.date.accessioned2020-12-21T01:46:55Z
dc.date.available2020-12-21T01:46:55Z
dc.date.issued2016-01-01
dc.identifier.citationGurung, I., Spielman, I., Davies, M. R., Lala, R., Gaustad, P., Biais, N. & Pelicic, V. (2016). Functional analysis of an unusual type IV pilus in the Gram-positive Streptococcus sanguinis. MOLECULAR MICROBIOLOGY, 99 (2), pp.380-392. https://doi.org/10.1111/mmi.13237.
dc.identifier.issn0950-382X
dc.identifier.urihttp://hdl.handle.net/11343/256656
dc.description.abstractType IV pili (Tfp), which have been studied extensively in a few Gram-negative species, are the paradigm of a group of widespread and functionally versatile nano-machines. Here, we performed the most detailed molecular characterisation of Tfp in a Gram-positive bacterium. We demonstrate that the naturally competent Streptococcus sanguinis produces retractable Tfp, which like their Gram-negative counterparts can generate hundreds of piconewton of tensile force and promote intense surface-associated motility. Tfp power 'train-like' directional motion parallel to the long axis of chains of cells, leading to spreading zones around bacteria grown on plates. However, S. sanguinis Tfp are not involved in DNA uptake, which is mediated by a related but distinct nano-machine, and are unusual because they are composed of two pilins in comparable amounts, rather than one as normally seen. Whole genome sequencing identified a locus encoding all the genes involved in Tfp biology in S. sanguinis. A systematic mutational analysis revealed that Tfp biogenesis in S. sanguinis relies on a more basic machinery (only 10 components) than in Gram-negative species and that a small subset of four proteins dispensable for pilus biogenesis are essential for motility. Intriguingly, one of the piliated mutants that does not exhibit spreading retains microscopic motility but moves sideways, which suggests that the corresponding protein controls motion directionality. Besides establishing S. sanguinis as a useful new model for studying Tfp biology, these findings have important implications for our understanding of these widespread filamentous nano-machines.
dc.languageEnglish
dc.publisherWILEY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleFunctional analysis of an unusual type IV pilus in the Gram-positive Streptococcus sanguinis
dc.typeJournal Article
dc.identifier.doi10.1111/mmi.13237
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titleMolecular Microbiology
melbourne.source.volume99
melbourne.source.issue2
melbourne.source.pages380-392
dc.rights.licenseCC BY
melbourne.elementsid1098298
melbourne.contributor.authorDavies, Mark
dc.identifier.eissn1365-2958
melbourne.accessrightsOpen Access


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