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dc.contributor.authorRaina, J-B
dc.contributor.authorTapiolas, D
dc.contributor.authorMotti, CA
dc.contributor.authorForet, S
dc.contributor.authorSeemann, T
dc.contributor.authorTebben, J
dc.contributor.authorWillis, BL
dc.contributor.authorBourne, DG
dc.date.accessioned2020-12-21T01:55:46Z
dc.date.available2020-12-21T01:55:46Z
dc.date.issued2016-08-18
dc.identifierpii: 2275
dc.identifier.citationRaina, J. -B., Tapiolas, D., Motti, C. A., Foret, S., Seemann, T., Tebben, J., Willis, B. L. & Bourne, D. G. (2016). Isolation of an antimicrobial compound produced by bacteria associated with reef-building corals. PEERJ, 4 (8), https://doi.org/10.7717/peerj.2275.
dc.identifier.issn2167-8359
dc.identifier.urihttp://hdl.handle.net/11343/256717
dc.description.abstractBacterial communities associated with healthy corals produce antimicrobial compounds that inhibit the colonization and growth of invasive microbes and potential pathogens. To date, however, bacteria-derived antimicrobial molecules have not been identified in reef-building corals. Here, we report the isolation of an antimicrobial compound produced by Pseudovibrio sp. P12, a common and abundant coral-associated bacterium. This strain was capable of metabolizing dimethylsulfoniopropionate (DMSP), a sulfur molecule produced in high concentrations by reef-building corals and playing a role in structuring their bacterial communities. Bioassay-guided fractionation coupled with nuclear magnetic resonance (NMR) and mass spectrometry (MS), identified the antimicrobial as tropodithietic acid (TDA), a sulfur-containing compound likely derived from DMSP catabolism. TDA was produced in large quantities by Pseudovibrio sp., and prevented the growth of two previously identified coral pathogens, Vibrio coralliilyticus and V. owensii, at very low concentrations (0.5 μg/mL) in agar diffusion assays. Genome sequencing of Pseudovibrio sp. P12 identified gene homologs likely involved in the metabolism of DMSP and production of TDA. These results provide additional evidence for the integral role of DMSP in structuring coral-associated bacterial communities and underline the potential of these DMSP-metabolizing microbes to contribute to coral disease prevention.
dc.languageEnglish
dc.publisherPEERJ INC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleIsolation of an antimicrobial compound produced by bacteria associated with reef-building corals
dc.typeJournal Article
dc.identifier.doi10.7717/peerj.2275
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titlePeerJ
melbourne.source.titlePeerJ PrePrints
melbourne.source.volume4
melbourne.source.issue8
dc.rights.licenseCC BY
melbourne.elementsid1106563
melbourne.contributor.authorSeemann, Torsten
dc.identifier.eissn2167-8359
melbourne.accessrightsOpen Access


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